Application
Fig1: Sample: HL-60 Cell (Human) Lysate at 40 ug Primary: Anti- E-Selectin (ER1908-35) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 65 kD Observed band size: 65 kDApplication
Fig2: Sample: Liver (Mouse) Lysate at 40 ug Primary: Anti- E-Selectin (ER1908-35) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 65 kD Observed band size: 65 kDApplication
Fig3: Sample: DU145 Cell Lysate at 40 ug LOVO Cell Lysate at 40 ug Primary: Anti-E-Selectin (wt-p53)(ER1908-35) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 65 kD Observed band size: 63 kDApplication
Fig4: Sample: Siha Cell Lysate at 40 ug Primary: Anti- E-Selectin (ER1908-35) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 65 kD Observed band size: 65 kD| Product Name | E-Selectin |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CD 62E CD26E CD62 antigen-like family member E CD62E CD62E antigen E selectin E-selectin ELAM 1 ELAM ELAM-1 ELAM1 Endothelial adhesion molecule 1 Endothelial leukocyte adhesion molecule 1 ESEL LECAM 2 LECAM2 Leukocyte endothelial cell adhesion molecule 2 Leukocyte-endothelial cell adhesion molecule 2 LYAM2_HUMAN SELE Selectin E SelectinE. |
| Product description | The protein encoded by this gene is found in cytokine-stimulated endothelial cells and is thought to be responsible for the accumulation of blood leukocytes at sites of inflammation by mediating the adhesion of cells to the vascular lining. It exhibits structural features such as the presence of lectin- and EGF-like domains followed by short consensus repeat (SCR) domains that contain 6 conserved cysteine residues. These proteins are part of the selectin family of cell adhesion molecules. Adhesion molecules participate in the interaction between leukocytes and the endothelium and appear to be involved in the pathogenesis of atherosclerosis. |
| Immunogen | KLH conjugated synthetic peptide derived from mouse E-Selectin 501-612/612 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatChickenDogPigHorseRabbit |
| Concentration | 1mg/ml |
| Alternative Names | CD 62E CD26E CD62 antigen-like family member E CD62E CD62E antigen E selectin E-selectin ELAM 1 ELAM ELAM-1 ELAM1 Endothelial adhesion molecule 1 Endothelial leukocyte adhesion molecule 1 ESEL LECAM 2 LECAM2 Leukocyte endothelial cell adhesion molecule 2 Leukocyte-endothelial cell adhesion molecule 2 LYAM2_HUMAN SELE Selectin E SelectinE. |
|---|---|
| Molecular Weight(MW) | 65 KDa |
| Cellular Localization | Membrane; Single-pass type I membrane protein. |
| SwissProt ID | Q00690 |
|---|
Application
Fig1: Sample: HL-60 Cell (Human) Lysate at 40 ug Primary: Anti- E-Selectin (ER1908-35) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 65 kD Observed band size: 65 kD
Application
Fig2: Sample: Liver (Mouse) Lysate at 40 ug Primary: Anti- E-Selectin (ER1908-35) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 65 kD Observed band size: 65 kD
Application
Fig3: Sample: DU145 Cell Lysate at 40 ug LOVO Cell Lysate at 40 ug Primary: Anti-E-Selectin (wt-p53)(ER1908-35) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 65 kD Observed band size: 63 kD
Application
Fig4: Sample: Siha Cell Lysate at 40 ug Primary: Anti- E-Selectin (ER1908-35) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 65 kD Observed band size: 65 kD| Positive Control | HL-60 Cell, Liver (Mouse) Lysate, DU145 Cell Lysate, Siha Cell Lysate. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
