Application
Fig1: Sample: A431(Human) Cell Lysate at 30 ug Hela(Human) Cell Lysate at 30 ug HepG2(Human) Cell Lysate at 30 ug Primary: Anti-ESRP2 (ER1908-36) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 78 kD Observed band size: 78 kDApplication
Fig2: Paraformaldehyde-fixed, paraffin embedded (Mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (ESRP2) Polyclonal Antibody, Unconjugated (ER1908-36) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Product Name | ESRP2 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Epithelial splicing regulatory protein 2 ESRP 2 ESRP2 ESRP2_HUMAN FLJ21918 FLJ22248 RBM35B RNA binding motif protein 35A RNA binding motif protein 35B RNA binding protein 35B RNA-binding motif protein 35B RNA-binding protein 35B. |
| Product description | mRNA splicing factor that regulates the formation of epithelial cell-specific isoforms. Specifically regulates the expression of FGFR2-IIIb, an epithelial cell-specific isoform of FGFR2. Also regulates the splicing of CD44, CTNND1, ENAH, 3 transcripts that undergo changes in splicing during the epithelial-to-mesenchymal transition (EMT). Acts by directly binding specific sequences in mRNAs. Binds the GU-rich sequence motifs in the ISE/ISS-3, a cis-element regulatory region present in the mRNA of FGFR2. |
| Immunogen | KLH conjugated synthetic peptide derived from human ESRP2 21-120/727 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatDogCow |
| Concentration | 1mg/ml |
| Alternative Names | Epithelial splicing regulatory protein 2 ESRP 2 ESRP2 ESRP2_HUMAN FLJ21918 FLJ22248 RBM35B RNA binding motif protein 35A RNA binding motif protein 35B RNA binding protein 35B RNA-binding motif protein 35B RNA-binding protein 35B. |
|---|---|
| Molecular Weight(MW) | 78 KDa |
| Cellular Localization | Nucleus. |
| SwissProt ID | Q9H6T0 |
|---|
Application
Fig1: Sample: A431(Human) Cell Lysate at 30 ug Hela(Human) Cell Lysate at 30 ug HepG2(Human) Cell Lysate at 30 ug Primary: Anti-ESRP2 (ER1908-36) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 78 kD Observed band size: 78 kD
Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (Mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (ESRP2) Polyclonal Antibody, Unconjugated (ER1908-36) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Positive Control | Epithelial cell-specific. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
