Application
Fig1: Sample: HepG2(Human) Cell Lysate at 30 ug Primary: Anti-Estrogen Sulfotransferase (ER1908-37) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 32 kD Observed band size: 32 kD| Product Name | Estrogen Sulfotransferase |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Estrogen Sulfotransferase Ste SULT1E1 Estrogen sulfotransferase, testis isoform Sulfotransferase, estrogen-preferring EST EST1 STE sulfotransferase family 1E, estrogen-preferring, member 1 Sulfotransferase estrogen preferring ST1E1_HUMAN. |
| Product description | Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfate conjugation of estradiol and estrone. May play a role in the regulation of estrogen receptor activity by metabolizing free estradiol. Maximally sulfates beta-estradiol and estrone at concentrations of 20 nM. Also sulfates dehydroepiandrosterone, pregnenolone, ethinylestradiol, equalenin, diethylstilbesterol and 1-naphthol, at significantly higher concentrations; however, cortisol, testosterone and dopamine are not sulfated. Sulfotransferase enzymes catalyze the sulfate conjugation of many hormones, neurotransmitters, drugs, and xenobiotic compounds. These cytosolic enzymes are different in their tissue distributions and substrate specificities. The gene structure (number and length of exons) is similar among family members. This gene encodes a protein that transfers a sulfo moiety to and from estrone, which may control levels of estrogen receptors. |
| Immunogen | KLH conjugated synthetic peptide derived from human Estrogen Sulfotranferase 51-145/294 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatDogPigHorseRabbit |
| Concentration | 1mg/ml |
| Alternative Names | Estrogen Sulfotransferase
Ste
SULT1E1
Estrogen sulfotransferase testis isoform Sulfotransferase estrogen-preferring EST EST1 STE sulfotransferase family 1E estrogen-preferring member 1 Sulfotransferase estrogen preferring ST1E1_HUMAN. |
|---|---|
| Molecular Weight(MW) | 32 KDa |
| Cellular Localization | Cytoplasm. |
| SwissProt ID | P49888 |
|---|
Application
Fig1: Sample: HepG2(Human) Cell Lysate at 30 ug Primary: Anti-Estrogen Sulfotransferase (ER1908-37) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 32 kD Observed band size: 32 kD| Positive Control | Liver, intestine and at lower level in the kidney. |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
