Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (ESYT1) Polyclonal Antibody, Unconjugated (ER1908-38) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig2: Sample: 293T(Human) Cell Lysate at 40 ug Primary: Anti-ESYT1 (ER1908-38) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 123 kD Observed band size: 123 kD| Product Name | ESYT1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Extended synaptotagmin 1 KIAA0747 E Syt1 E-Syt1 Esyt1 ESYT1_HUMAN Extended synaptotagmin like protein 1 Extended synaptotagmin-1 Family with sequence similarity 62 (C2 domain containing) member A Family with sequence similarity 62 member A MBC2 Membrane bound C2 domain containing protein Membrane-bound C2 domain-containing protein Protein FAM62A. |
| Product description | May play a role as calcium-regulated intrinsic membrane protein. |
| Immunogen | KLH conjugated synthetic peptide derived from human ESYT1/FAM62A 651-750/1104 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatDogPigCowHorseRabbitMonkey |
| Concentration | 1mg/ml |
| Alternative Names | Extended synaptotagmin 1 KIAA0747 E Syt1 E-Syt1 Esyt1 ESYT1_HUMAN Extended synaptotagmin like protein 1 Extended synaptotagmin-1 Family with sequence similarity 62 (C2 domain containing) member A Family with sequence similarity 62 member A MBC2 Membrane bound C2 domain containing protein Membrane-bound C2 domain-containing protein Protein FAM62A. |
|---|---|
| Molecular Weight(MW) | 123 KDa |
| Cellular Localization | Membrane. Localizes to intracellular membranes. |
| SwissProt ID | Q9BSJ8 |
|---|
Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (ESYT1) Polyclonal Antibody, Unconjugated (ER1908-38) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig2: Sample: 293T(Human) Cell Lysate at 40 ug Primary: Anti-ESYT1 (ER1908-38) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 123 kD Observed band size: 123 kD| Positive Control | Widely expressed. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
