Application
Fig1: Sample: 293T(Human) Cell Lysate at 30 ug Primary: Anti-EZH1 (ER1908-44) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 85 kD Observed band size: 76 kDApplication
Fig2: Paraformaldehyde-fixed, paraffin embedded (Rat lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (EZH1) Polyclonal Antibody, Unconjugated (ER1908-44) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig3: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (EZH1) Polyclonal Antibody, Unconjugated (ER1908-44) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Product Name | EZH1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Enhancer of zeste homolog 1(Drosophila) Enhancer of zeste homolog 1 ENX-2 ENX2 EZH1 EZH1: enhancer of zeste homolog 1 (Drosophila) EZH1_HUMAN Histone-lysine N-methyltransferase EZH1 KIAA0388. |
| Product description | Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH1 complex, which methylates 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Required for embryonic stem cell derivation and self-renewal, suggesting that it is involved in safeguarding embryonic stem cell identity. Compared to EZH1-containing complexes, it is less abundant in embryonic stem cells, has weak methyltransferase activity and plays a less critical role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. |
| Immunogen | KLH conjugated synthetic peptide derived from human EZH1 471-510/747 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatChickenDogPigCowRabbitGuinea Pig |
| Concentration | 1mg/ml |
| Alternative Names | Enhancer of zeste homolog 1(Drosophila) Enhancer of zeste homolog 1 ENX-2 ENX2 EZH1 EZH1: enhancer of zeste homolog 1 (Drosophila) EZH1_HUMAN Histone-lysine N-methyltransferase EZH1 KIAA0388. |
|---|---|
| Molecular Weight(MW) | 85 KDa |
| Cellular Localization | Nucleus. Note=Colocalizes with trimethylated 'Lys-27' of histone H3. |
| SwissProt ID | Q92800 |
|---|
Application
Fig1: Sample: 293T(Human) Cell Lysate at 30 ug Primary: Anti-EZH1 (ER1908-44) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 85 kD Observed band size: 76 kD
Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (Rat lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (EZH1) Polyclonal Antibody, Unconjugated (ER1908-44) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig3: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (EZH1) Polyclonal Antibody, Unconjugated (ER1908-44) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Positive Control | 293T(Human) Cell Lysate, Rat lung, Mouse brain. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
