Application
Fig1: Sample: Raji (human)cell Lysate at 30 ug Primary: Anti- Factor VIIIa light chain (ER1908-48)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 75kD Observed band size: 85kDApplication
Fig2: Sample: MCF-7 (human)cell Lysate at 30 ug Primary: Anti- Factor VIIIa light chain (ER1908-48)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 75kD Observed band size: 75kDApplication
Fig3: Sample: Lymph node (Mouse) Lysate at 40 ug Testis (Mouse) Lysate at 40 ug Primary: Anti-Factor VIIIa light chain (ER1908-48) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 75 kD Observed band size: 75 kD| Product Name | Factor VIIIa light chain |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | coagulation factor VIII Ahf Antihemophilic factor Coagulation factor VIII Coagulation factor VIII associated protein b Coagulation factor VIII isoform b Coagulation factor VIII procoagulent component Coagulation factor VIIIc Dna segment on chromosome x unique 1253 expressed sequence Dxs1253e F8 F8 protein F8b F8c FactorVIII FVIII Hema Hema coagulation factor VIIIc procoagulent component Hemophilia a Hemophilia classic OTTHUMP00000061446 Procoagulant component AHF DXS1253E F8B F8C FVIII HEMA FA8_HUMAN. |
| Product description | Factor VIII, along with calcium and phospholipid, acts as a cofactor for factor IXa when it converts factor X to the activated form, factor Xa. |
| Immunogen | KLH conjugated synthetic peptide derived from human Factor VIIIa light chain 1751-1850/2351 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Alternative Names | coagulation factor VIII Ahf Antihemophilic factor Coagulation factor VIII Coagulation factor VIII associated protein b Coagulation factor VIII isoform b Coagulation factor VIII procoagulent component Coagulation factor VIIIc Dna segment on chromosome x unique 1253 expressed sequence Dxs1253e F8 F8 protein F8b F8c FactorVIII FVIII Hema Hema coagulation factor VIIIc procoagulent component Hemophilia a Hemophilia classic OTTHUMP00000061446 Procoagulant component AHF DXS1253E F8B F8C FVIII HEMA FA8_HUMAN. |
|---|---|
| Molecular Weight(MW) | 75 KDa |
| Cellular Localization | Secreted, extracellular space. |
| SwissProt ID | P00451 |
|---|
Application
Fig1: Sample: Raji (human)cell Lysate at 30 ug Primary: Anti- Factor VIIIa light chain (ER1908-48)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 75kD Observed band size: 85kD
Application
Fig2: Sample: MCF-7 (human)cell Lysate at 30 ug Primary: Anti- Factor VIIIa light chain (ER1908-48)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 75kD Observed band size: 75kD
Application
Fig3: Sample: Lymph node (Mouse) Lysate at 40 ug Testis (Mouse) Lysate at 40 ug Primary: Anti-Factor VIIIa light chain (ER1908-48) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 75 kD Observed band size: 75 kD| Application Notes | WB:1:500-2000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
