Application
Fig1: Sample: 293T Cell (Human) Lysate at 40 ug Primary: Anti-Factor XII heavy chain (ER1908-50) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 40/68 kD Observed band size: 38 kDApplication
Fig2: Sample: HepG2 Cell (Human) Lysate at 40 ug Primary: Anti-Factor XII heavy chain (ER1908-50) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 40/68 kD Observed band size: 38 kDApplication
Fig3: Sample: U937(Human) Cell Lysate at 30 ug Primary: Anti- Factor XIIa heavy chain (ER1908-50) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 40/68 kD Observed band size: 68 kD| Product Name | Factor XIIa heavy chain |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Factor XII Coagulation factor XII Factor XII heavy chain Coagulation factor XIIa heavy chain F12 F12 deficiency FA12_HUMAN Factor XII deficiency HAE3 HAEX HAF HAF deficiency Hageman factor Coagulation factor XIIa heavy chain. |
| Product description | Factor XII is a serum glycoprotein that participates in the initiation of blood coagulation, fibrinolysis, and the generation of bradykinin and angiotensin. Prekallikrein is cleaved by factor XII to form kallikrein, which then cleaves factor XII first to alpha-factor XIIa and then trypsin cleaves it to beta-factor XIIa. Alpha-factor XIIa activates factor XI to factor XIa. |
| Immunogen | KLH conjugated synthetic peptide derived from human Coagulation factor XIIa heavy chain 31-130/615 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatDogCowHorseSheep |
| Concentration | 1mg/ml |
| Alternative Names | Factor XII Coagulation factor XII Factor XII heavy chain Coagulation factor XIIa heavy chain F12 F12 deficiency FA12_HUMAN Factor XII deficiency HAE3 HAEX HAF HAF deficiency Hageman factor Coagulation factor XIIa heavy chain. |
|---|---|
| Molecular Weight(MW) | 40/68 KDa |
| Cellular Localization | Secreted. |
| SwissProt ID | P00748 |
|---|
Application
Fig1: Sample: 293T Cell (Human) Lysate at 40 ug Primary: Anti-Factor XII heavy chain (ER1908-50) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 40/68 kD Observed band size: 38 kD
Application
Fig2: Sample: HepG2 Cell (Human) Lysate at 40 ug Primary: Anti-Factor XII heavy chain (ER1908-50) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 40/68 kD Observed band size: 38 kD
Application
Fig3: Sample: U937(Human) Cell Lysate at 30 ug Primary: Anti- Factor XIIa heavy chain (ER1908-50) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 40/68 kD Observed band size: 68 kD| Application Notes | WB:1:500-2000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
