Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (FADD) Polyclonal Antibody, Unconjugated (ER1908-51) at 1:400 overnight at 4℃, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining.Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (FADD) Polyclonal Antibody, Unconjugated (ER1908-51) at 1:400 overnight at 4℃, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining.Application
Fig3: Sample: A431(Human) Cell Lysate at 30 ug Primary: Anti-FADD (ER1908-51) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 23 kD Observed band size: 23 kDApplication
Fig4: Sample: A431(Human) Cell Lysate at 30 ug Primary: Anti-FADD (ER1908-51) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 23 kD Observed band size: 23 kD| Product Name | FADD |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | FADD protein Fas-associated protein with death domain Fas associated via death domain Fas associating death domain containing protein Fas associating protein Fas associating protein with death domain FasTNFRSF6 associated via death domain GIG 3 GIG3 Growth inhibiting gene 3 protein H sapiens mRNA for mediator of receptor induced toxicity Mediator of receptor induced toxicity MGC8528 MORT1 FADD_HUMAN. |
| Product description | Apoptotic adaptor molecule that recruits caspase-8 or caspase-10 to the activated Fas (CD95) or TNFR-1 receptors. The resulting aggregate called the death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation. Active caspase-8 initiates the subsequent cascade of caspases mediating apoptosis. |
| Immunogen | KLH conjugated synthetic peptide derived from human FADD 1-80/205 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatPig |
| Concentration | 1mg/ml |
| Alternative Names | FADD protein Fas-associated protein with death domain Fas associated via death domain Fas associating death domain containing protein Fas associating protein Fas associating protein with death domain FasTNFRSF6 associated via death domain GIG 3 GIG3 Growth inhibiting gene 3 protein H sapiens mRNA for mediator of receptor induced toxicity Mediator of receptor induced toxicity MGC8528 MORT1 FADD_HUMAN. |
|---|---|
| Molecular Weight(MW) | 23 KDa |
| SwissProt ID | Q13158 |
|---|
Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (FADD) Polyclonal Antibody, Unconjugated (ER1908-51) at 1:400 overnight at 4℃, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining.
Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (FADD) Polyclonal Antibody, Unconjugated (ER1908-51) at 1:400 overnight at 4℃, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining.
Application
Fig3: Sample: A431(Human) Cell Lysate at 30 ug Primary: Anti-FADD (ER1908-51) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 23 kD Observed band size: 23 kD
Application
Fig4: Sample: A431(Human) Cell Lysate at 30 ug Primary: Anti-FADD (ER1908-51) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 23 kD Observed band size: 23 kD| Positive Control | Expressed in a wide variety of tissues, except for peripheral blood mononuclear leukocytes. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
