Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (FAM3C) Polyclonal Antibody, Unconjugated (ER1908-63) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig2: Sample: U87MG(Human) Cell Lysate at 30 ug Primary: Anti-FAM3C (ER1908-63) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 22 kD Observed band size: 22 kDApplication
Fig3: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (FAM3C) Polyclonal Antibody, Unconjugated (ER1908-63) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Product Name | FAM3C |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Fam3c FAM3C_HUMAN family with sequence similarity 3, member C GS3786 ILEI Interleukin-like EMT inducer Predicted osteoblast protein Protein FAM3C. |
| Product description | May be involved in retinal laminar formation. Promotes epithelial to mesenchymal transition. |
| Immunogen | KLH conjugated synthetic peptide derived from human FAM3C 151-227/227 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatChickenDogPigCowHorse |
| Concentration | 1mg/ml |
| Alternative Names | Fam3c
FAM3C_HUMAN
family with sequence similarity 3 member C GS3786 ILEI Interleukin-like EMT inducer Predicted osteoblast protein Protein FAM3C. |
|---|---|
| Molecular Weight(MW) | 22 KDa |
| Cellular Localization | Secreted. Cytoplasmic vesicle. Note=Cytoplasmic in some cancer cells. |
| SwissProt ID | Q92520 |
|---|
Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (FAM3C) Polyclonal Antibody, Unconjugated (ER1908-63) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig2: Sample: U87MG(Human) Cell Lysate at 30 ug Primary: Anti-FAM3C (ER1908-63) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 22 kD Observed band size: 22 kD
Application
Fig3: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (FAM3C) Polyclonal Antibody, Unconjugated (ER1908-63) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Positive Control | Present in most secretory epithelia (at protein level). |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
