F box only protein 25
F box protein 25
F box protein Fbx25
F-box only protein 25
FBX25
MGC20256
MGC51975
OTTHUMP00000115399
Product description
Substrate-recognition component of the SCF(SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex. Mayplay a role in accumulation of expanded polyglutamine (polyQ)protein huntingtin (HTT) (By similarity).
Immunogen
KLH conjugated synthetic peptide derived from human FBXO25 55-150/367
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
WB
WB:1:500-2000:
Species Reactivity
HumanMouseRatPigRabbit
Concentration
1mg/ml
Target Information
Alternative Names
F box only protein 25
F box protein 25
F box protein Fbx25
F-box only protein 25
FBX25
MGC20256
MGC51975
OTTHUMP00000115399
Molecular Weight(MW)
43 KDa
Cellular Localization
Nuclear. Note=In the nucleus, associateswith a subnuclear dot-like structure. Colocalized with SKP1
Fig1: Sample:
Hippocampus (Mouse) Lysate at 40 ug
Primary: Anti-FBXO25 (ER1908-73) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 43 kD
Observed band size: 43 kD
Positive Control
Expressed in all brain tissue observed.
Application Notes
WB:1:500-2000:
Additional Information
Form
Liquid
Storage Instructions
Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
Storage Buffer
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.