| Product Name | FBXW8 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | F box/WD repeat containing protein 8 F box and WD40 domain containing protein 8 F-box only protein 29 F box only protein 29 FBW6 FBW8 FBX29 FBXO29 FBXW6 FBXW8_HUMAN. |
| Product description | Substrate-recognition component of a Cul7-RING ubiquitin-protein ligase complex, which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. The Cul7-RING(FBXW8) complex mediates ubiquitination and consequent degradation of GORASP1, acting as a component of the ubiquitin ligase pathway that regulates Golgi morphogenesis and dendrite patterning in brain. Mediates ubiquitination and degradation of IRS1 in a mTOR-dependent manner: the Cul7-RING(FBXW8) complex recognizes and binds IRS1 previously phosphorylated by S6 kinase (RPS6KB1 or RPS6KB2). The Cul7-RING(FBXW8) complex also mediates ubiquitination of MAP4K1/HPK1: recognizes and binds autophosphorylated MAP4K1/HPK1, leading to its degradation, thereby affecting cell proliferation and differentiation. Associated component of the 3M complex, suggesting that it mediates some of 3M complex functions. |
| Immunogen | KLH conjugated synthetic peptide derived from human FBXW8/FBXO29 181-280/598 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatDogPigCowHorseSheep |
| Concentration | 1mg/ml |
| Alternative Names | F box/WD repeat containing protein 8 F box and WD40 domain containing protein 8 F-box only protein 29 F box only protein 29 FBW6 FBW8 FBX29 FBXO29 FBXW6 FBXW8_HUMAN. |
|---|---|
| Molecular Weight(MW) | 67 KDa |
| Cellular Localization | Golgi apparatus. |
| SwissProt ID | Q8N3Y1 |
|---|
| Positive Control | U87-MG, SK-N-SH and IMR32 whole cell lysates. |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
