Application
Fig1: Sample: Cerebrum (Mouse) Lysate at 40 ug Primary: Anti- FE65 (ER1908-79) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 77 kD Observed band size: 75 kDApplication
Fig2: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (FE65) Polyclonal Antibody, Unconjugated (ER1908-79) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig3: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (FE65) Polyclonal Antibody, Unconjugated (ER1908-79) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig4: Sample: Cerebrum (Rat) Lysate at 40 ug Primary: Anti- FE65 (ER1908-79) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 77 kD Observed band size: 75 kD| Product Name | FE65 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Adaptor protein FE65a2 Amyloid beta (A4) precursor protein binding family B member 1 Amyloid beta A4 precursor protein binding family B Amyloid beta A4 precursor protein binding family B member 1 Amyloid beta precursor protein binding family B member 1 APBB 1 APBB1 FE 65 Fe65 protein MGC 9072 MGC9072 Protein Fe65 RIR Stat like protein Fe-65 Protein APBB1_HUMAN. |
| Product description | Transcription coregulator that can have both coactivator and corepressor functions. Adapter protein that forms a transcriptionally active complex with the gamma-secretase-derived amyloid precursor protein (APP) intracellular domain. Plays a central role in the response to DNA damage by translocating to the nucleus and inducing apoptosis. May act by specifically recognizing and binding histone H2AX phosphorylated on 'Tyr-142' (H2AXY142ph) at double-strand breaks (DSBs), recruiting other pro-apoptosis factors such as MAPK8/JNK1. Required for histone H4 acetylation at double-strand breaks (DSBs). Its ability to specifically bind modified histones and chromatin modifying enzymes such as KAT5/TIP60, probably explains its trancription activation activity. Function in association with TSHZ3, SET and HDAC factors as a transcriptional repressor, that inhibits the expression of CASP4. Associates with chromatin in a region surrounding the CASP4 transcriptional start site(s). |
| Immunogen | KLH conjugated synthetic peptide derived from human Fe65 protein 201-300/710 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatRabbit |
| Concentration | 1mg/ml |
| Alternative Names | Adaptor protein FE65a2 Amyloid beta (A4) precursor protein binding family B member 1 Amyloid beta A4 precursor protein binding family B Amyloid beta A4 precursor protein binding family B member 1 Amyloid beta precursor protein binding family B member 1 APBB 1 APBB1 FE 65 Fe65 protein MGC 9072 MGC9072 Protein Fe65 RIR Stat like protein Fe-65 Protein APBB1_HUMAN. |
|---|---|
| Molecular Weight(MW) | 77 KDa |
| Cellular Localization | Cell membrane. Cytoplasm. Nucleus. Cell projection, growth cone. Nucleus speckle. Note=Colocalizes with TSHZ3 in axonal growth cone. In normal conditions, it mainly localizes to the cytoplasm, while a small fraction is tethered to the cell membrane via its interaction with APP. Following exposure to DNA damaging agents, it is released from cell membrane and translocates to the nucleus. Nuclear translocation is under the regulation of APP. Colocalizes with TSHZ3 in the nucleus. Co-localizes with NEK6 at the nuclear speckles. Phosphorylation at Ser-610 by SGK1 promotes its localization to the nucleus. |
| SwissProt ID | O00213 |
|---|
Application
Fig1: Sample: Cerebrum (Mouse) Lysate at 40 ug Primary: Anti- FE65 (ER1908-79) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 77 kD Observed band size: 75 kD
Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (FE65) Polyclonal Antibody, Unconjugated (ER1908-79) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig3: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (FE65) Polyclonal Antibody, Unconjugated (ER1908-79) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig4: Sample: Cerebrum (Rat) Lysate at 40 ug Primary: Anti- FE65 (ER1908-79) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 77 kD Observed band size: 75 kD| Positive Control | Highly expressed in brain; strongly reduced in post-mortem elderly subjects with Alzheimer disease. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
