Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by microwave in sodium citratebuffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3%BSA) at RTfor 30min; Antibody incubation with (FGF5) Polyclonal/MonoclonalAntibody, Unconjugated (ER1908-83) at 1:400 overnight at 4℃,followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by microwave in sodium citratebuffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3%BSA) at RTfor 30min; Antibody incubation with (FGF5) Polyclonal/MonoclonalAntibody, Unconjugated (ER1908-83) at 1:400 overnight at 4℃,followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.| Product Name | FGF5 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | FGF 5 FGF-5 FGF5 FGF5_HUMAN fibroblast growth factor 5 HBGF 5 HBGF-5 heparin binding growth factor 5 Heparin-binding growth factor 5 Smag 82 Smag-82. |
| Product description | Functions as an inhibitor of hair elongation by promoting progression from anagen, the growth phase of the hair follicle, into catagen the apoptosis-induced regression phase. |
| Immunogen | KLH conjugated synthetic peptide derived from human FGF5 151-268/268 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatChickenDogCow |
| Concentration | 1mg/ml |
| Alternative Names | FGF 5 FGF-5 FGF5 FGF5_HUMAN fibroblast growth factor 5 HBGF 5 HBGF-5 heparin binding growth factor 5 Heparin-binding growth factor 5 Smag 82 Smag-82. |
|---|---|
| Molecular Weight(MW) | 78 KDa |
| Cellular Localization | Secreted. |
| SwissProt ID | P12034 |
|---|
Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by microwave in sodium citratebuffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3%BSA) at RTfor 30min; Antibody incubation with (FGF5) Polyclonal/MonoclonalAntibody, Unconjugated (ER1908-83) at 1:400 overnight at 4℃,followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by microwave in sodium citratebuffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3%BSA) at RTfor 30min; Antibody incubation with (FGF5) Polyclonal/MonoclonalAntibody, Unconjugated (ER1908-83) at 1:400 overnight at 4℃,followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.| Positive Control | Expressed in neonatal brain. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
