Application
Fig1: Sample:U251(Human)Cell Lysate at 40 ug Primary: Anti-FLNB(ER1908-91)at 1/300 dilution Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution Predicted band size: 278kD Observed band size: 278kDApplication
Fig2: Sample:Hela (Human)Cell Lysate at 40 ug Primary: Anti-FLNB(ER1908-91)at 1/300 dilution Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution Predicted band size: 278kD Observed band size: 278kD| Product Name | FLNB |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ABP 278 ABP 280 homolog ABP-278 ABP-280 homolog ABP278 Actin binding like protein Actin binding protein 278 Actin-binding-like protein AOI Beta filamin Beta-filamin DKFZp686A1668 DKFZp686O033 Fh1 Filamin 1 actin binding protein 280 like Filamin 3 Filamin B Filamin B beta actin binding protein 278 Filamin homolog 1 Filamin-3 Filamin-B FLN B FLN-B FLN1L FLN3 FLNB FLNB_HUMAN LRS1 SCT TABP TAP Thyroid autoantigen Truncated ABP Truncated actin binding protein Truncated actin-binding protein. |
| Product description | Connects cell membrane constituents to the actin cytoskeleton. May promote orthogonal branching of actin filaments and links actin filaments to membrane glycoproteins. Anchors various transmembrane proteins to the actin cytoskeleton. Interaction with FLNA may allow neuroblast migration from the ventricular zone into the cortical plate. Various interactions and localizations of isoforms affect myotube morphology and myogenesis. Isoform 6 accelerates muscle differentiation in vitro. |
| Immunogen | KLH conjugated synthetic peptide derived from human FLNB/Filamin 3 1551-1650/2602 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRatDogCowRabbitSheep |
| Concentration | 1mg/ml |
| Alternative Names | ABP 278 ABP 280 homolog ABP-278 ABP-280 homolog ABP278 Actin binding like protein Actin binding protein 278 Actin-binding-like protein AOI Beta filamin Beta-filamin DKFZp686A1668 DKFZp686O033 Fh1 Filamin 1 actin binding protein 280 like Filamin 3 Filamin B Filamin B beta actin binding protein 278 Filamin homolog 1 Filamin-3 Filamin-B FLN B FLN-B FLN1L FLN3 FLNB FLNB_HUMAN LRS1 SCT TABP TAP Thyroid autoantigen Truncated ABP Truncated actin binding protein Truncated actin-binding protein. |
|---|---|
| Molecular Weight(MW) | 278 KDa |
| Cellular Localization | Isoform 1: Cytoplasm, cell cortex. Cytoplasm, cytoskeleton. Cytoplasm, myofibril, sarcomere, Z line. Note=In differentiating myotubes, isoform 1, isoform 2 and isoform 3 are localized diffusely throughout the cytoplasm with regions of enrichment at the longitudinal actin stress fiber. In differentiated tubes, isoform 1 is also detected within the Z-lines. ;Isoform 2: Cytoplasm, cytoskeleton. Note=Predominantly localized at actin stress fibers. ;Isoform 3: Cytoplasm, cytoskeleton. Note=Predominantly localized at actin stress fibers. ;Isoform 6: Cytoplasm, cytoskeleton. Note=Polarized at the periphery of myotubes. |
| SwissProt ID | O75369 |
|---|
Application
Fig1: Sample:U251(Human)Cell Lysate at 40 ug Primary: Anti-FLNB(ER1908-91)at 1/300 dilution Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution Predicted band size: 278kD Observed band size: 278kD
Application
Fig2: Sample:Hela (Human)Cell Lysate at 40 ug Primary: Anti-FLNB(ER1908-91)at 1/300 dilution Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution Predicted band size: 278kD Observed band size: 278kD| Positive Control | Ubiquitous. Isoform 1 and isoform 2 are expressed in placenta, bone marrow, brain, umbilical vein endothelial cells (HUVEC), retina and skeletal muscle. Isoform 1 is predominantly expressed in prostate, uterus, liver, thyroid, stomach, lymph node, small intestine, spleen, skeletal muscle, kidney, placenta, pancreas, heart, lung, platelets, endothelial cells, megakaryocytic and erythroleukemic cell lines. Isoform 2 is predominantly expressed in spinal cord, platelet and Daudi cells. Also expressed in thyroid adenoma, neurofibrillary tangles (NFT), senile plaques in the hippocampus and cerebral cortex in Alzheimer disease (AD). Isoform 3 and isoform 6 are expressed predominantly in lung, heart, skeletal muscle, testis, spleen, thymus and leukocytes. Isoform 4 and isoform 5 are expressed in heart. |
|---|---|
| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
