Application
Fig1: Sample: HepG2(Human) Cell Lysate at 30 ug NIH/3T3(Mouse) Cell Lysate at 30 ug K562(Human) Lysate at 30 ug Heart (Mouse) Lysate at 40 ug Primary: Anti-FNDC5 (ER1908-96) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 12/20 kD Observed band size: 20 kDApplication
Fig2: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (FNDC5) Polyclonal Antibody, Unconjugated (ER1908-96) at 1:500 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.| Product Name | FNDC5 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | FNDC5 Fibronectin type III domain containing 5 Fibronectin type III domain-containing protein 5 Fibronectin type III repeat containing protein 2 Fibronectin type III repeat-containing protein 2 FNDC 5 Fndc5 FNDC5_HUMAN FRCP2 Irisin. |
| Product description | Irisin: mediates beneficial effects of muscular exercise. Induces browning of white adipose tissue by stimulating UCP1 expression, at least in part, via the nuclear receptor PPARA (By similarity). |
| Immunogen | KLH conjugated synthetic peptide derived from human FNDC5 Irisin 81-143/212 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatChickenDogRabbit |
| Concentration | 1mg/ml |
| Alternative Names | FNDC5 Fibronectin type III domain containing 5 Fibronectin type III domain-containing protein 5 Fibronectin type III repeat containing protein 2 Fibronectin type III repeat-containing protein 2 FNDC 5 Fndc5 FNDC5_HUMAN FRCP2 Irisin. |
|---|---|
| Molecular Weight(MW) | 43819 KDa |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein. Peroxisome membrane; Single-pass type I membrane protein (By similarity). Note=Imported in peroxisomes through the PEX5 receptor pathway (By similarity). Irisin: Secreted. Note=Detected in the blood of individuals subjected to endurance exercise. |
| SwissProt ID | Q8NAU1 |
|---|
Application
Fig1: Sample: HepG2(Human) Cell Lysate at 30 ug NIH/3T3(Mouse) Cell Lysate at 30 ug K562(Human) Lysate at 30 ug Heart (Mouse) Lysate at 40 ug Primary: Anti-FNDC5 (ER1908-96) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 12/20 kD Observed band size: 20 kD
Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (FNDC5) Polyclonal Antibody, Unconjugated (ER1908-96) at 1:500 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
