Application
Fig1: Protein: embryo(mouse) lysates at 30ug; colon carcinoma(human) lysates at 30ug; Primary: Anti-Frizzled 10/CD350 (ER1909-02) at 1:200; Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000; ECL excitated the fluorescence; Predicted band size : 68kD Observed band size : 68kD| Product Name | Frizzled 10/CD350 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CD 350 CD 350 antigen CD350 Frizzled (Drosophila) homolog 10 Frizzled-10 FZ 10 Fz-10 FZ10 FZD 10 FZD10 FZD10_HUMAN FzE 7 FzE7 hFz 10 hFz10. |
| Product description | Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. |
| Immunogen | KLH conjugated synthetic peptide derived from human Frizzled 10/CD350 101-200/581 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatChickenDogPigCowSheep |
| Concentration | 1mg/ml |
| Alternative Names | CD 350 CD 350 antigen CD350 Frizzled (Drosophila) homolog 10 Frizzled-10 FZ 10 Fz-10 FZ10 FZD 10 FZD10 FZD10_HUMAN FzE 7 FzE7 hFz 10 hFz10. |
|---|---|
| Molecular Weight(MW) | 68 KDa |
| Cellular Localization | Cell membrane. |
| SwissProt ID | Q9ULW2 |
|---|
Application
Fig1: Protein: embryo(mouse) lysates at 30ug; colon carcinoma(human) lysates at 30ug; Primary: Anti-Frizzled 10/CD350 (ER1909-02) at 1:200; Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000; ECL excitated the fluorescence; Predicted band size : 68kD Observed band size : 68kD| Positive Control | Highest levels in the placenta and fetal kidney, followed by fetal lung and brain. In adult brain, abundantly expressed in the cerebellum, followed by cerebral cortex, medulla and spinal cord; very low levels in total brain, frontal lobe, temporal lobe and putamen. Weak expression detected in adult brain, heart, lung, skeletal muscle, pancreas, spleen and prostate. |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
