Application
Fig1: Sample: HepG2(Human) Cell Lysate at 30 ug Muscle (Mouse) Lysate at 40 ug Muscle (Rat) Lysate at 40 ug Primary: Anti-Frizzled 7 (ER1909-03) at 1/2000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 60 kD Observed band size: 60 kDApplication
Fig2: Blank control: A431. Primary Antibody (green line): Rabbit Anti-Frizzled 7 antibody (ER1909-03) Dilution: 3μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody: Goat anti-rabbit IgG-AF647 Dilution: 3μg /test. Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.Application
Fig3: Sample: Muscle (Mouse) Lysate at 40 ug Primary: Anti-Frizzled 7 (ER1909-03) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 60 kD Observed band size: 60 kD| Product Name | Frizzled 7 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Frizzled drosophila homolog of 7 Frizzled homolog 7 Frizzled7 Fz 7 Fz7 FZD 7 FZD7 FzE 3 FZE3 Frizzled 3 Frizzled3 Frizzled-3 hFz 7 hFz7 Homolog of Drosophila Frizzled 7. |
| Product description | Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. |
| Immunogen | KLH conjugated synthetic peptide derived from human Frizzled 7 501-574/574 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:500-2000: FC:3ug/Test: |
|
| Species Reactivity | HumanMouseRatChickenDogPigCowGuinea Pig |
| Concentration | 1mg/ml |
| Alternative Names | Frizzled drosophila homolog of 7 Frizzled homolog 7 Frizzled7 Fz 7 Fz7 FZD 7 FZD7 FzE 3 FZE3 Frizzled 3 Frizzled3 Frizzled-3 hFz 7 hFz7 Homolog of Drosophila Frizzled 7. |
|---|---|
| Molecular Weight(MW) | 60 KDa |
| Cellular Localization | Membrane; Multi-pass membrane protein. |
| SwissProt ID | O75084 |
|---|
Application
Fig1: Sample: HepG2(Human) Cell Lysate at 30 ug Muscle (Mouse) Lysate at 40 ug Muscle (Rat) Lysate at 40 ug Primary: Anti-Frizzled 7 (ER1909-03) at 1/2000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 60 kD Observed band size: 60 kD
Application
Fig2: Blank control: A431. Primary Antibody (green line): Rabbit Anti-Frizzled 7 antibody (ER1909-03) Dilution: 3μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody: Goat anti-rabbit IgG-AF647 Dilution: 3μg /test. Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Application
Fig3: Sample: Muscle (Mouse) Lysate at 40 ug Primary: Anti-Frizzled 7 (ER1909-03) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 60 kD Observed band size: 60 kD| Positive Control | High expression in adult skeletal muscle and fetal kidney, followed by fetal lung, adult heart, brain, and placenta. Specifically expressed in squamous cell esophageal carcinomas. |
|---|---|
| Application Notes | WB:1:500-2000: FC:3ug/Test: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
