Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (FSD1) Polyclonal Antibody, Unconjugated (ER1909-06) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (FSD1) Polyclonal Antibody, Unconjugated (ER1909-06) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Product Name | FSD1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Fibronectin type 3 and SPRY (spla, ryanodine) domain containing (with coiled-coil motif) 1 Fibronectin type 3 and SPRY domain containing 1 Fibronectin type III and SPRY domain containing 1 Fibronectin type III and SPRY domain-containing protein 1 Fibronectin type III and SPRY domains containing protein 1 FSD1 FSD1_HUMAN GLFND MGC3213 MGC58341 Microtubule-associated protein GLFND MID1-related protein 1 midline 1-related protein 1 MIR1 VLP27. |
| Product description | May be involved in microtubule organization and stabilization. |
| Immunogen | KLH conjugated synthetic peptide derived from human FSD1 421-496/496 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatDogPigCowHorseSheep |
| Concentration | 1mg/ml |
| Alternative Names | Fibronectin type 3 and SPRY (spla ryanodine) domain containing (with coiled-coil motif) 1 Fibronectin type 3 and SPRY domain containing 1 Fibronectin type III and SPRY domain containing 1 Fibronectin type III and SPRY domain-containing protein 1 Fibronectin type III and SPRY domains containing protein 1 FSD1 FSD1_HUMAN GLFND MGC3213 MGC58341 Microtubule-associated protein GLFND MID1-related protein 1 midline 1-related protein 1 MIR1 VLP27. |
|---|---|
| Molecular Weight(MW) | 56 KDa |
| Cellular Localization | Cytoplasm > cytoskeleton > centrosome. Nucleus. Cytoplasm. Cleavage furrow. Cell-cycle-dependent association with the centrosome. Colocalizes with a subpopulation of microtubules. Does not associates with microtubules during mitosis but reassociates with microtubules during cytokinesis. Localizes to the central portions of a small subset of microtubules in interphase cells and a subpopulation of microtubules in the cleavage furrow, not present in the mitotic spindle. |
| SwissProt ID | Q9BTV5 |
|---|
Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (FSD1) Polyclonal Antibody, Unconjugated (ER1909-06) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (FSD1) Polyclonal Antibody, Unconjugated (ER1909-06) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Positive Control | Highly expressed in brain tissues, including cerebellum, cerebral cortex, medulla, occipital pole, frontal lobe, temporal lobe and putamen. Lower expression in spinal chord. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
