Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (rat ovary); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (FSH receptor ) Polyclonal Antibody, Unconjugated (ER1909-08) at 1:400 overnight at 4℃, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining.Application
Fig2: Sample: Ovary (mouse) Lysate at 40 ug Primary: Anti- FSH receptor (ER1909-08)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 78kD Observed band size: 78kDApplication
Fig3: Blank control (blue line): MCF7 (fixed with 70% methanol overnight at 4℃). Primary Antibody (green line): Rabbit Anti-FSH receptor antibody (ER1909-08) ,Dilution: 0.2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE,Dilution: 1μg /test.| Product Name | FSH receptor |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Follicle-stimulating hormone receptor precursor Follicle stimulating hormone receptor Follicle stimulating hormone receptor isoform 1 Follitropin receptor FSHRO LGR1 ODG1 Follicle-stimulating hormone receptor FSH-R FSH R Fshr FSHR_HUMAN MGC141667 MGC141668 ovarian dysgenesis 1. |
| Product description | The FSHR consists of 695 amino acids and has a molecular mass of about 76 kDa. Like other GPCRs, the FSH-receptor possesses seven membrane-spanning domains or transmembrane helices. The extracellular domain of the receptor contains 11 leucine-rich repeats and is glycosylated. It has two subdomains, a hormone-binding subdomain followed by a signal-specificity subdomain. The hormone-binding subdomain is responsible for the high-affinity hormone binding, and the signal-specificity subdomain, containing a sulfated tyrosine at position 335 (sTyr) in a hinge loop, is required for the hormone activity. The transmembrane domain contains two highly conserved cysteine residues that build disulfide bonds to stabilize the receptor structure. A highly conserved Asp-Arg-Tyr triplet motif is present in GPCR family members in general and may be of importance to transmit the signal. In FSHR and its closely related other glycoprotein hormone receptor members (LHR and TSHR), this conserved triplet motif is a variation Glu-Arg-Trp sequence. |
| Immunogen | KLH conjugated synthetic peptide derived from human FSH receptor 21-100/695 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-PFC |
| WB:1:500-2000: IHC-P:1:400-800: FC:1ug/Test: |
|
| Species Reactivity | HumanMouseRatDogPigCowHorseSheep |
| Concentration | 1mg/ml |
| Alternative Names | Follicle-stimulating hormone receptor precursor
Follicle stimulating hormone receptor
Follicle stimulating hormone receptor isoform 1 Follitropin receptor FSHRO LGR1 ODG1 Follicle-stimulating hormone receptor FSH-R FSH R Fshr FSHR_HUMAN MGC141667 MGC141668 ovarian dysgenesis 1. |
|---|---|
| Molecular Weight(MW) | 78 KDa |
| Cellular Localization | Cell membrane; Multi-pass membrane protein. |
| SwissProt ID | P23945 |
|---|
Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (rat ovary); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (FSH receptor ) Polyclonal Antibody, Unconjugated (ER1909-08) at 1:400 overnight at 4℃, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining.
Application
Fig2: Sample: Ovary (mouse) Lysate at 40 ug Primary: Anti- FSH receptor (ER1909-08)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 78kD Observed band size: 78kD
Application
Fig3: Blank control (blue line): MCF7 (fixed with 70% methanol overnight at 4℃). Primary Antibody (green line): Rabbit Anti-FSH receptor antibody (ER1909-08) ,Dilution: 0.2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE,Dilution: 1μg /test.| Positive Control | Sertoli cells and ovarian granulosa cells. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: FC:1ug/Test: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
