Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (rat testis tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (FYTTD1) Polyclonal Antibody, Unconjugated (ER1909-15) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig2: Sample: Hela(Human) Cell Lysate at 30 ug Primary: Anti- FYTTD1 (ER1909-15) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 36 kD Observed band size: 34 kD| Product Name | FYTTD1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DKFZp761B1514 Forty two three domain containing 1 Forty two three domain containing protein 1 Forty-two-three domain-containing protein 1 FYTTD 1 fyttd1 OTTHUMP00000208028 OTTHUMP00000208031 OTTHUMP00000208032 Protein 40 2 3 Protein 40-2-3 UAP56 interacting factor UAP56-interacting factor UIF UIF_HUMAN. |
| Product description | Required for mRNA export from the nucleus to the cytoplasm. Acts as an adapter that uses the DDX39B/UAP56-NFX1 pathway to ensure efficient mRNA export and delivering to the nuclear pore. Associates with spliced and unspliced mRNAs simultaneously with THOC4. |
| Immunogen | KLH conjugated synthetic peptide derived from human FYTTD1 210-318/318 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatDogPig |
| Concentration | 1mg/ml |
| Alternative Names | DKFZp761B1514 Forty two three domain containing 1 Forty two three domain containing protein 1 Forty-two-three domain-containing protein 1 FYTTD 1 fyttd1 OTTHUMP00000208028 OTTHUMP00000208031 OTTHUMP00000208032 Protein 40 2 3 Protein 40-2-3 UAP56 interacting factor UAP56-interacting factor UIF UIF_HUMAN. |
|---|---|
| Molecular Weight(MW) | 36 KDa |
| Cellular Localization | Nucleus; nucleoplasm. Nucleus speckle. |
| SwissProt ID | Q96QD9 |
|---|
Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (rat testis tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (FYTTD1) Polyclonal Antibody, Unconjugated (ER1909-15) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig2: Sample: Hela(Human) Cell Lysate at 30 ug Primary: Anti- FYTTD1 (ER1909-15) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 36 kD Observed band size: 34 kD| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
