Application
Fig1: Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-FAD3 Polyclonal Antibody, Unconjugated(ER1909-16) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingApplication
Fig2: Protein: brain(Rat) lysates at 30ug; Primary: Anti-FZD3 (ER1909-16) at 1:300; Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000; ECL excitated the fluorescence; Predicted band size : 74kD Observed band size : 74kD, 25kDApplication
Fig3: Protein: pancreas(mouse) lysates at 30ug; Primary: Anti-FZD3 (ER1909-16) at 1:300; Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000; ECL excitated the fluorescence; Predicted band size : 74kD Observed band size : 74kDApplication
Fig4: Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-FZD3 Polyclonal Antibody, Unconjugated(ER1909-16) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingApplication
Fig5: Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-FZD3 Polyclonal Antibody, Unconjugated(ER1909-16) 1:500, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining| Product Name | FZD3 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Frizzled 3 Frizzled 3 seven transmembrane spanning receptor Frizzled family receptor 3 Frizzled homolog 3 (Drosophila) Frizzled homolog 3 Frizzled-3 Frizzled3 Fz 3 Fz-3 Fz3 FZD 3 Fzd3 FZD3_HUMAN hFz 3 hFz3. |
| Product description | Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. |
| Immunogen | KLH conjugated synthetic peptide derived from human FZD3/Frizzled 3 121-220/666 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatChickenDogPigCowHorseRabbitMonkeyXenopusGorillaOpossumMarmosetBatElephant |
| Concentration | 1mg/ml |
| Alternative Names | Frizzled 3 Frizzled 3 seven transmembrane spanning receptor Frizzled family receptor 3 Frizzled homolog 3 (Drosophila) Frizzled homolog 3 Frizzled-3 Frizzled3 Fz 3 Fz-3 Fz3 FZD 3 Fzd3 FZD3_HUMAN hFz 3 hFz3. |
|---|---|
| Molecular Weight(MW) | 74 KDa |
| Cellular Localization | Membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein. |
| SwissProt ID | Q9NPG1 |
|---|
Application
Fig1: Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-FAD3 Polyclonal Antibody, Unconjugated(ER1909-16) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Application
Fig2: Protein: brain(Rat) lysates at 30ug; Primary: Anti-FZD3 (ER1909-16) at 1:300; Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000; ECL excitated the fluorescence; Predicted band size : 74kD Observed band size : 74kD, 25kD
Application
Fig3: Protein: pancreas(mouse) lysates at 30ug; Primary: Anti-FZD3 (ER1909-16) at 1:300; Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000; ECL excitated the fluorescence; Predicted band size : 74kD Observed band size : 74kD
Application
Fig4: Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-FZD3 Polyclonal Antibody, Unconjugated(ER1909-16) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Application
Fig5: Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-FZD3 Polyclonal Antibody, Unconjugated(ER1909-16) 1:500, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining| Positive Control | Widely expressed. Relatively high expression in the CNS, including regions of the limbic system, in kidney, pancreas, skeletal muscle, uterus and testis. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
