Application
Fig1: Protein: intestinal(mouse) lysate at 40ug; Primary: rabbit Anti-FZR1 (ER1909-17) at 1:300; Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000; Predicted band size: 55 kD Observed band size: 55 kDApplication
Fig2: Sample: tetis (Mouse) Lysate at 40 ug Primary: Anti-FZR1(ER1909-17) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55 kD Observed band size: 55 kD| Product Name | FZR1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CDC20 like 1b CDC20 like protein 1 CDC20-like protein 1 CDC20C Cdh 1 CDH1 Cdh1/Hct1 homolog Fizzy related protein 1 Fizzy related protein 1 Fizzy related protein Fizzy related protein homolog Fizzy-related protein homolog Fizzy/cell division cycle 20 related 1 FYR FZR 1 FZR 2 FZR FZR_HUMAN FZR1 Fzr1 protein FZR2 HCDH 1 HCDH HCDH1 KIAA1242. |
| Product description | Key regulator of ligase activity of the anaphase promoting complex/cyclosome (APC/C), which confers substrate specificity upon the complex. Associates with the APC/C in late mitosis, in replacement of CDC20, and activates the APC/C during anaphase and telophase. The APC/C remains active in degrading substrates to ensure that positive regulators of the cell cycle do not accumulate prematurely. At the G1/S transition FZR1 is phosphorylated, leading to its dissociation from the APC/C. Following DNA damage, it is required for the G2 DNA damage checkpoint: its dephosphorylation and reassociation with the APC/C leads to the ubiquitination of PLK1, preventing entry into mitosis. |
| Immunogen | KLH conjugated synthetic peptide derived from human FZR1/CDC20C 141-240/496 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatChickenDogPigCowHorseSheep |
| Concentration | 1mg/ml |
| Alternative Names | CDC20 like 1b CDC20 like protein 1 CDC20-like protein 1 CDC20C Cdh 1 CDH1 Cdh1/Hct1 homolog Fizzy related protein 1 Fizzy related protein 1 Fizzy related protein Fizzy related protein homolog Fizzy-related protein homolog Fizzy/cell division cycle 20 related 1 FYR FZR 1 FZR 2 FZR FZR_HUMAN FZR1 Fzr1 protein FZR2 HCDH 1 HCDH HCDH1 KIAA1242. |
|---|---|
| Molecular Weight(MW) | 55 KDa |
| Cellular Localization | Cytoplasm and Nucleus. |
| SwissProt ID | Q9UM11 |
|---|
Application
Fig1: Protein: intestinal(mouse) lysate at 40ug; Primary: rabbit Anti-FZR1 (ER1909-17) at 1:300; Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000; Predicted band size: 55 kD Observed band size: 55 kD
Application
Fig2: Sample: tetis (Mouse) Lysate at 40 ug Primary: Anti-FZR1(ER1909-17) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55 kD Observed band size: 55 kD| Positive Control | Isoform 2 is expressed at high levels in heart, liver, spleen and some cancer cell lines whereas isoform 3 is expressed only at low levels in these tissues. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
