Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (G21 protein/NPR2L) Polyclonal Antibody, Unconjugated (ER1909-19) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig2: Sample: HepG2(Human) Cell Lysate at 30 ug Primary: Anti-NPR2L (ER1909-19) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 44 kD Observed band size: 44 kD| Product Name | G21 protein/NPR2L |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | G21 protein Gene 21 protein Homologous to yeast nitrogen permease (candidate tumor suppressor) Homologous to yeast nitrogen permease NPR 2L NPR L2 NPR like 2 NPR2 like NPRL 2 NPRL2 Tumor suppressor candidate 4 TUSC 4 TUSC 4 protein TUSC4 TUSC4 protein NPRL2_HUMAN. |
| Product description | Suppresses Src-dependent tyrosine phosphorylation and activation of PDPK1 and its downstream signaling. Down-regulates PDPK1 kinase activity by interfering with tyrosine phosphorylation at the Tyr-9 Tyr-373 and Tyr-376 residues. May act as a tumor suppressor. Suppresses cell growth and enhanced sensitivity to various anticancer drugs. |
| Immunogen | KLH conjugated synthetic peptide derived from human NPR2L 221-320/380 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatPigCowHorseRabbit |
| Concentration | 1mg/ml |
| Alternative Names | G21 protein Gene 21 protein Homologous to yeast nitrogen permease (candidate tumor suppressor) Homologous to yeast nitrogen permease NPR 2L NPR L2 NPR like 2 NPR2 like NPRL 2 NPRL2 Tumor suppressor candidate 4 TUSC 4 TUSC 4 protein TUSC4 TUSC4 protein NPRL2_HUMAN. |
|---|---|
| Molecular Weight(MW) | 44 KDa |
| SwissProt ID | Q8WTW4 |
|---|
Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (G21 protein/NPR2L) Polyclonal Antibody, Unconjugated (ER1909-19) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig2: Sample: HepG2(Human) Cell Lysate at 30 ug Primary: Anti-NPR2L (ER1909-19) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 44 kD Observed band size: 44 kD| Positive Control | Most abundant in skeletal muscle, followed by brain, liver and pancreas, with lower amounts in lung, kidney, placenta and heart. Expressed in most lung cancer cell lines tested. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
