Application
Fig1: Sample:A431 (human)cell Lysate at 40 ug Primary: Anti-GABA Transporter 3 (ER1909-22)at 1/300 dilution Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution Predicted band size: 71 kD Observed band size: 71 kDApplication
Fig2: Blank control: HepG2. Primary Antibody (green line): Rabbit Anti-GABA Transporter 3 antibody (ER1909-22) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.| Product Name | GABA Transporter 3 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GABT3 GAT-3 GAT3 S6A11_HUMAN Slc6a11 Sodium- and chloride-dependent GABA transporter 3 SOLUTE CARRIER FAMILY 6 (NEUROTRANSMITTER TRANSPORTER, GABA), MEMBER 11 Solute carrier family 6 member 11. |
| Product description | Terminates the action of GABA by its high affinity sodium-dependent reuptake into presynaptic terminals. |
| Immunogen | KLH conjugated synthetic peptide derived from human GABA Transporter 3/GAT 3 531-632/632 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:500-2000: FC:1ug/test: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | GABT3
GAT-3
GAT3
S6A11_HUMAN
Slc6a11
Sodium- and chloride-dependent GABA transporter 3
SOLUTE CARRIER FAMILY 6 (NEUROTRANSMITTER TRANSPORTER GABA) MEMBER 11 Solute carrier family 6 member 11. |
|---|---|
| Molecular Weight(MW) | 71 KDa |
| Cellular Localization | Membrane; Multi-pass membrane protein. |
| SwissProt ID | P48066 |
|---|
Application
Fig1: Sample:A431 (human)cell Lysate at 40 ug Primary: Anti-GABA Transporter 3 (ER1909-22)at 1/300 dilution Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution Predicted band size: 71 kD Observed band size: 71 kD
Application
Fig2: Blank control: HepG2. Primary Antibody (green line): Rabbit Anti-GABA Transporter 3 antibody (ER1909-22) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.| Positive Control | Widespread distribution in the brain. |
|---|---|
| Application Notes | WB:1:500-2000: FC:1ug/test: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
