Application
Fig1: Sample: 293T Cell (Human) Lysate at 30 ug Primary: Anti- GABPA (ER1909-23)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 51kD Observed band size: 51kDApplication
Fig2: Paraformaldehyde-fixed, paraffin embedded (Rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (GABPA) Polyclonal Antibody, Unconjugated (ER1909-23) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Product Name | GABPA |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | E4TF 1A E4TF1 60 E4TF1A GA binding protein alpha chain GA binding protein transcription factor alpha subunit 60kDa GA binding protein transcription factor alpha subunit GA-binding protein alpha chain GABP A GABP alpha subunit GABP alpha subunit GABP subunit alpha Gabpa GABPA_HUMAN Human nuclear respiratory factor 2 subunit alpha NFT 2 NFT2 NRF 2 NRF 2A NRF2 NRF2A Nuclear respiratory factor 2 alpha subunit Nuclear respiratory factor 2 subunit alpha Nuclear respiratory factor 2 subunit alpha RCH04A07 Transcription factor E4TF1 60 Transcription factor E4TF1 60 Transcription factor E4TF1-60. |
| Product description | This gene encodes one of three GA-binding protein transcription factor subunits which functions as a DNA-binding subunit. Since this subunit shares identity with a subunit encoding the nuclear respiratory factor 2 gene, it is likely involved in activation of cytochrome oxidase expression and nuclear control of mitochondrial function. This subunit also shares identity with a subunit constituting the transcription factor E4TF1, responsible for expression of the adenovirus E4 gene. Because of its chromosomal localization and ability to form heterodimers with other polypeptides, this gene may play a role in the Down Syndrome phenotype. Two transcript variants encoding the same protein have been found for this gene. |
| Immunogen | KLH conjugated synthetic peptide derived from human GABPA 51-150/454 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatChickenDogPigCowRabbitSheep |
| Concentration | 1mg/ml |
| Alternative Names | E4TF 1A E4TF1 60 E4TF1A GA binding protein alpha chain GA binding protein transcription factor alpha subunit 60kDa GA binding protein transcription factor alpha subunit GA-binding protein alpha chain GABP A GABP alpha subunit GABP alpha subunit GABP subunit alpha Gabpa GABPA_HUMAN Human nuclear respiratory factor 2 subunit alpha NFT 2 NFT2 NRF 2 NRF 2A NRF2 NRF2A Nuclear respiratory factor 2 alpha subunit Nuclear respiratory factor 2 subunit alpha Nuclear respiratory factor 2 subunit alpha RCH04A07 Transcription factor E4TF1 60 Transcription factor E4TF1 60 Transcription factor E4TF1-60. |
|---|---|
| Molecular Weight(MW) | 51 KDa |
| Cellular Localization | Nucleus. |
| SwissProt ID | Q06546 |
|---|
Application
Fig1: Sample: 293T Cell (Human) Lysate at 30 ug Primary: Anti- GABPA (ER1909-23)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 51kD Observed band size: 51kD
Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (Rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (GABPA) Polyclonal Antibody, Unconjugated (ER1909-23) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Positive Control | 293T Cell, Rat kidney tissue. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
