Application
Fig1: Blank control(blue):RSC96 (fixed with 2% paraformaldehyde (10 min)). Primary Antibody:Rabbit Anti- GABR B3 antibody(ER1909-24), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.Application
Fig2: Sample: Cerebrum (Mouse) Lysate at 40 ug Primary: Anti-GABR B3 (ER1909-24) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52 kD Observed band size: 48 kDApplication
Fig3: Sample: Cerebrum (Mouse) Lysate at 40 ug Hippocampus (Mouse) Lysate at 40 ug Primary: Anti-GABR B3 (ER1909-24) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52 kD Observed band size: 52 kDApplication
Fig4: Sample: Cerebrum (Rat) Lysate at 40 ug Primary: Anti-GABR B3 (ER1909-24) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52 kD Observed band size: 52 kD| Product Name | GABR B3 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GABA A Receptor beta 3 ECA5 GABA alpha receptor beta-2 subunit GABA(A) receptor subunit beta-3 GABAA receptor beta 3 subunit GABAA receptor subunit beta 3 GABR B3 Gabrb3 Gamma aminobutyric acid (GABA) A receptor beta 3 Gamma aminobutyric acid receptor subunit beta 3 Gamma-aminobutyric acid receptor subunit beta-3 GBRB3_HUMAN MGC9051. |
| Product description | Component of the heteropentameric receptor for GABA, the major inhibitory neurotransmitter in the vertebrate brain. Functions also as histamine receptor and mediates cellular responses to histamine. Functions as receptor for diazepines and various anesthetics, such as pentobarbital; these are bound at a separate allosteric effector binding site. Functions as ligand-gated chloride channel. |
| Immunogen | KLH conjugated synthetic peptide derived from human GABR B3/GABA A Receptor beta 3 31-130/473 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:500-2000: FC:1μg/Test: |
|
| Species Reactivity | HumanMouseRatChickenPigCowHorse |
| Concentration | 1mg/ml |
| Alternative Names | GABA A Receptor beta 3 ECA5 GABA alpha receptor beta-2 subunit GABA(A) receptor subunit beta-3 GABAA receptor beta 3 subunit GABAA receptor subunit beta 3 GABR B3 Gabrb3 Gamma aminobutyric acid (GABA) A receptor beta 3 Gamma aminobutyric acid receptor subunit beta 3 Gamma-aminobutyric acid receptor subunit beta-3 GBRB3_HUMAN MGC9051. |
|---|---|
| Molecular Weight(MW) | 52 KDa |
| Cellular Localization | Plasma membrane |
| SwissProt ID | P28472 |
|---|
Application
Fig1: Blank control(blue):RSC96 (fixed with 2% paraformaldehyde (10 min)). Primary Antibody:Rabbit Anti- GABR B3 antibody(ER1909-24), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Application
Fig2: Sample: Cerebrum (Mouse) Lysate at 40 ug Primary: Anti-GABR B3 (ER1909-24) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52 kD Observed band size: 48 kD
Application
Fig3: Sample: Cerebrum (Mouse) Lysate at 40 ug Hippocampus (Mouse) Lysate at 40 ug Primary: Anti-GABR B3 (ER1909-24) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52 kD Observed band size: 52 kD
Application
Fig4: Sample: Cerebrum (Rat) Lysate at 40 ug Primary: Anti-GABR B3 (ER1909-24) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52 kD Observed band size: 52 kD| Application Notes | WB:1:500-2000: FC:1μg/Test: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
