Application
Fig1: Sample: Cerebrum (Mouse) Lysate at 40 ug Primary: Anti-GABRA2 (ER1909-25) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 48 kD Observed band size: 48 kDApplication
Fig2: Sample: Cerebral cortex(Rat) Lysate at 40 ug Primary: Anti-GABRA2 (ER1909-25) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 48 kD Observed band size: 48 kDApplication
Fig3: Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-GABRA2 Polyclonal Antibody, Unconjugated(ER1909-25) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingApplication
Fig4: Sample: Cerebrum (mouse) Lysate at 40 ug Primary: Anti- GABRA2(ER1909-25) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 48 kD Observed band size: 50 kDApplication
Fig5: Blank control(blue):RSC96 (fixed with 2% paraformaldehyde (10 min)). Primary Antibody:Rabbit Anti- GABRA2 antibody(ER1909-25), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.| Product Name | GABRA2 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GABA A Receptor a2 GABA A Receptor alpha 2 GABA A receptor subunit alpha 2 GABA GABA(A) receptor subunit alpha 2 GABA(A) receptor subunit alpha-2 GABR A2 GABRA 2 GABRA2 GABRA2 protein Gamma aminobutyric acid (GABA) A receptor alpha 2 Gamma aminobutyric acid A receptor alpha 2 Gamma aminobutyric acid receptor alpha 2 subunit Gamma aminobutyric acid receptor subunit alpha 2 Gamma-aminobutyric acid receptor subunit alpha-2 GBRA2_HUMAN. |
| Product description | GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel. |
| Immunogen | KLH conjugated synthetic peptide derived from human GABRA2/GABA A Receptor alpha 2 185-280/451 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-PFC |
| WB:1:500-2000: IHC-P:1:400-800: FC:1μg/Test: |
|
| Species Reactivity | HumanMouseRatDogPigCowHorseRabbit |
| Concentration | 1mg/ml |
| Alternative Names | GABA A Receptor a2 GABA A Receptor alpha 2 GABA A receptor subunit alpha 2 GABA GABA(A) receptor subunit alpha 2 GABA(A) receptor subunit alpha-2 GABR A2 GABRA 2 GABRA2 GABRA2 protein Gamma aminobutyric acid (GABA) A receptor alpha 2 Gamma aminobutyric acid A receptor alpha 2 Gamma aminobutyric acid receptor alpha 2 subunit Gamma aminobutyric acid receptor subunit alpha 2 Gamma-aminobutyric acid receptor subunit alpha-2 GBRA2_HUMAN. |
|---|---|
| Molecular Weight(MW) | 48 KDa |
| Cellular Localization | Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein. |
| SwissProt ID | P47869 |
|---|
Application
Fig1: Sample: Cerebrum (Mouse) Lysate at 40 ug Primary: Anti-GABRA2 (ER1909-25) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 48 kD Observed band size: 48 kD
Application
Fig2: Sample: Cerebral cortex(Rat) Lysate at 40 ug Primary: Anti-GABRA2 (ER1909-25) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 48 kD Observed band size: 48 kD
Application
Fig3: Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-GABRA2 Polyclonal Antibody, Unconjugated(ER1909-25) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Application
Fig4: Sample: Cerebrum (mouse) Lysate at 40 ug Primary: Anti- GABRA2(ER1909-25) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 48 kD Observed band size: 50 kD
Application
Fig5: Blank control(blue):RSC96 (fixed with 2% paraformaldehyde (10 min)). Primary Antibody:Rabbit Anti- GABRA2 antibody(ER1909-25), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.| Application Notes | WB:1:500-2000: IHC-P:1:400-800: FC:1μg/Test: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
