Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (GABRB2) Polyclonal Antibody, Unconjugated (ER1909-26) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig2: Sample: Cerebellum (rat) Lysate at 40 ug Cerebellum (mouse) Lysate at 40 ug Primary: Anti- GABRB2 (ER1909-26)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 56kD Observed band size: 56 kD| Product Name | GABRB2 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GABA A Receptor beta 2 GABA GABA(A) receptor subunit beta-2 GABRB2 Gamma aminobutyric acid (GABA) A receptor, beta 2 Gamma aminobutyric acid A receptor beta 2 Gamma-aminobutyric acid receptor subunit beta-2 Gamma-aminobutyric-acid receptor subunit beta-2 GBRB2_HUMAN, |
| Product description | GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel. |
| Immunogen | KLH conjugated synthetic peptide derived from human GABRB2/GABA A Receptor beta 2 251-350/512 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatChickenDogPigCowHorseRabbitSheep |
| Concentration | 1mg/ml |
| Alternative Names | GABA A Receptor beta 2
GABA
GABA(A) receptor subunit beta-2
GABRB2
Gamma aminobutyric acid (GABA) A receptor beta 2 Gamma aminobutyric acid A receptor beta 2 Gamma-aminobutyric acid receptor subunit beta-2 Gamma-aminobutyric-acid receptor subunit beta-2 GBRB2_HUMAN |
|---|---|
| Molecular Weight(MW) | 56 KDa |
| Cellular Localization | Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein. |
| SwissProt ID | P47870 |
|---|
Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (GABRB2) Polyclonal Antibody, Unconjugated (ER1909-26) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig2: Sample: Cerebellum (rat) Lysate at 40 ug Cerebellum (mouse) Lysate at 40 ug Primary: Anti- GABRB2 (ER1909-26)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 56kD Observed band size: 56 kD| Positive Control | Isoform 1 and isoform 2 show reduced expression in schizophrenic brain. Isoform 3 shows increased expression in schizophrenic and bipolar disorder brains while isoform 4 shows reduced expression. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
