Application
Fig1: Sample:Raji(human) Cell Lysate at 40 ug Primary: Anti-GALNT11(ER1909-33)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 69kD Observed band size: 69kDApplication
Fig2: Sample:siha(human) Cell Lysate at 40 ug Primary: Anti-GALNT11(ER1909-33)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 69kD Observed band size: 69kDApplication
Fig3: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (GALNT11) Polyclonal Antibody, Unconjugated (ER1909-33) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Product Name | GALNT11 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | A430075I06Rik AI648252 E430002F06Rik FLJ21634 GalNAc-T11 GALNT11 GLT11_HUMAN MGC71630 Polypeptide GalNAc transferase 11 Polypeptide N-acetylgalactosaminyltransferase 11 pp-GaNTase 11 Protein-UDP acetylgalactosaminyltransferase 11 tcag7.1057 UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 11. |
| Product description | Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. Displays the same enzyme activity toward Muc1, Muc4.1, and EA2 than GALNT1. Does not appear to be involved in glycosylation of erythropoietin. |
| Immunogen | KLH conjugated synthetic peptide derived from human GALNT11/GalNAc-T11 401-500/608 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatDogHorseRabbit |
| Concentration | 1mg/ml |
| Alternative Names | A430075I06Rik AI648252 E430002F06Rik FLJ21634 GalNAc-T11 GALNT11 GLT11_HUMAN MGC71630 Polypeptide GalNAc transferase 11 Polypeptide N-acetylgalactosaminyltransferase 11 pp-GaNTase 11 Protein-UDP acetylgalactosaminyltransferase 11 tcag7.1057 UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 11. |
|---|---|
| Molecular Weight(MW) | 69 KDa |
| Cellular Localization | Golgi apparatus membrane. |
| SwissProt ID | Q8NCW6 |
|---|
Application
Fig1: Sample:Raji(human) Cell Lysate at 40 ug Primary: Anti-GALNT11(ER1909-33)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 69kD Observed band size: 69kD
Application
Fig2: Sample:siha(human) Cell Lysate at 40 ug Primary: Anti-GALNT11(ER1909-33)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 69kD Observed band size: 69kD
Application
Fig3: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (GALNT11) Polyclonal Antibody, Unconjugated (ER1909-33) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Positive Control | Highly expressed in kidney. Expressed at intermediate level in brain, heart and skeletal muscle. Weakly expressed other tissues. In kidney, it is strongly expressed in tubules but not expressed in glomeruli. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
