Application
Fig1: Tissue/cell: Rat brian; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti- Gamma-Adaptin, Unconjugated(ER1909-36) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingApplication
Fig2: Tissue/cell: rat lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Gamma-Adaptin Polyclonal Antibody, Unconjugated(ER1909-36) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingApplication
Fig3: Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Gamma-Adaptin Polyclonal Antibody, Unconjugated(ER1909-36) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingApplication
Fig4: Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Gamma-Adaptin) Polyclonal Antibody, Unconjugated (ER1909-36) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig5: Sample: 293T(Human) Cell Lysate at 30 ug Jurkat(Human) Cell Lysate at 30 ug Primary: Anti-Gamma-Adaptin (ER1909-36) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 91 kD Observed band size: 91 kD| Product Name | Gamma-Adaptin |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Adapter-related protein complex 1 subunit gamma-1 Adaptor protein complex AP 1 gamma 1 subunit Adaptor protein complex AP 1 subunit gamma 1 Adaptor protein complex AP-1 subunit gamma-1 Adaptor related protein complex 1 gamma 1 subunit adaptor-related protein complex 1 gamma 1 subunit ADTG ADTG AP 1 complex subunit gamma 1 AP-1 complex subunit gamma-1 AP1G1 AP1G1_HUMAN CLAPG1 CLAPG1 Clathrin assembly protein complex 1 gamma 1 large chain Clathrin assembly protein complex 1 gamma large chain Clathrin assembly protein complex 1 gamma-1 large chain Clathrin associated/assembly/adaptor protein large gamma 1 clathrin-associated/assembly/adaptor protein large gamma 1 Gamma1 adaptin Gamma1-adaptin Golgi adaptor HA1/AP1 adaptin gamma subunit Golgi adaptor HA1/AP1 adaptin subunit gamma 1 Golgi adaptor HA1/AP1 adaptin subunit gamma-1 MGC18255. |
| Product description | Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules. |
| Immunogen | KLH conjugated synthetic peptide derived from human Gamma-Adaptin 721-822/822 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-PICC/IF |
| WB:1:500-2000: IHC-P:1:400-800: IF:1:100-500: ICC:1:100-500: |
|
| Species Reactivity | HumanMouseRatChickenDogPigCowRabbit |
| Concentration | 1mg/ml |
| Alternative Names | Adapter-related protein complex 1 subunit gamma-1 Adaptor protein complex AP 1 gamma 1 subunit Adaptor protein complex AP 1 subunit gamma 1 Adaptor protein complex AP-1 subunit gamma-1 Adaptor related protein complex 1 gamma 1 subunit adaptor-related protein complex 1 gamma 1 subunit ADTG ADTG AP 1 complex subunit gamma 1 AP-1 complex subunit gamma-1 AP1G1 AP1G1_HUMAN CLAPG1 CLAPG1 Clathrin assembly protein complex 1 gamma 1 large chain Clathrin assembly protein complex 1 gamma large chain Clathrin assembly protein complex 1 gamma-1 large chain Clathrin associated/assembly/adaptor protein large gamma 1 clathrin-associated/assembly/adaptor protein large gamma 1 Gamma1 adaptin Gamma1-adaptin Golgi adaptor HA1/AP1 adaptin gamma subunit Golgi adaptor HA1/AP1 adaptin subunit gamma 1 Golgi adaptor HA1/AP1 adaptin subunit gamma-1 MGC18255. |
|---|---|
| Molecular Weight(MW) | 91 KDa |
| Cellular Localization | Golgi apparatus. Cytoplasmic vesicle, clathrin-coated vesicle membrane; Peripheral membrane protein; Cytoplasmic side. |
| SwissProt ID | P22892 |
|---|
Application
Fig1: Tissue/cell: Rat brian; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti- Gamma-Adaptin, Unconjugated(ER1909-36) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Application
Fig2: Tissue/cell: rat lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Gamma-Adaptin Polyclonal Antibody, Unconjugated(ER1909-36) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Application
Fig3: Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Gamma-Adaptin Polyclonal Antibody, Unconjugated(ER1909-36) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Application
Fig4: Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Gamma-Adaptin) Polyclonal Antibody, Unconjugated (ER1909-36) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig5: Sample: 293T(Human) Cell Lysate at 30 ug Jurkat(Human) Cell Lysate at 30 ug Primary: Anti-Gamma-Adaptin (ER1909-36) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 91 kD Observed band size: 91 kD| Positive Control | Widely expressed. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: IF:1:100-500: ICC:1:100-500: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
