IHC
Tissue/cell: rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Incubation: Anti-GAPDHS Polyclonal Antibody 1:200, overnight at 4℃, followed by conjugation to the secondary antibody and DAB stainingWB
Sample: Testis (Mouse) Lysate at 40 ug, Primary: Anti-GAPDHS at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 45 kD, Observed band size: 47 kD.| Product Name | GAPDHS |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | May play an important role in regulating the switch between different pathways for energy production during spermiogenesis and in the spermatozoon. Required for sperm motility and male fertility. |
| Immunogen | KLH conjugated synthetic peptide derived from human GAPDHS 251-350/408 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:500-2000 IHC:1:400-800 |
|
| Species Reactivity | CowDogHorseHumanMouseRatSheep |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | GAPDH2
GAPD2
GAPDH 2
GAPDH 2
GAPDHS
GAPDS
Glyceraldehyde 3 phosphate dehydrogenase spermatogenic
Glyceraldehyde 3 phosphate dehydrogenase testis specific HSD 35 HSD35 Spermatogenic cell specific glyceraldehyde 3 phosphate dehydrogenase 2 Spermatogenic glyceraldehyde 3 phosphate dehydrogenase G3PT_HUMAN HSD-35. |
|---|---|
| Molecular Weight(MW) | 45 KDa |
| Cellular Localization | Cytoplasm. |
| SwissProt ID | O14556 |
|---|
IHC
Tissue/cell: rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Incubation: Anti-GAPDHS Polyclonal Antibody 1:200, overnight at 4℃, followed by conjugation to the secondary antibody and DAB staining
WB
Sample: Testis (Mouse) Lysate at 40 ug, Primary: Anti-GAPDHS at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 45 kD, Observed band size: 47 kD.| Positive Control | Testis specific. |
|---|---|
| Application Notes | WB:1:500-2000 IHC:1:400-800 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
