Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Mouse placenta); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (GDF15) Polyclonal Antibody, Unconjugated (ER1909-46) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.Application
Fig2: Sample: K562 Cell Lysate at 30 ug A431 Cell Lysate at 30 ug Primary: Anti-GDF15 (ER1909-46) at 1:300 dilution; Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000 dilution; Predicted band size:34 kD Observed band size:30 kD| Product Name | GDF15 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GDF 15 GDF-15 Growth differentiation factor 15 Growth/differentiation factor 15 Macrophage inhibitory cytokine 1 MIC 1 MIC-1 MIC1 NAG 1 NAG1 NRG 1 NRG1 NSAID (nonsteroidal anti inflammatory drug) activated protein 1 NSAID NSAID regulated protein 1 PDF PLAB Placental bone morphogenetic protein Placental bone morphogenic protein Placental TGF beta placental transformation growth factor beta Prostate differentiation factor PTGF beta PTGFB prostate derived factor. |
| Product description | Growth/differentiation factor 15 (GDF15) was first identified as Macrophage inhibitory cytokine-1 or MIC-1. It is a protein belonging to the transforming growth factor beta superfamily. Under normal conditions, GDF-15 is expressed in low concentrations in most organs and upregulated because of injury of organs such as such as liver, kidney, heart and lung. The function of GDF-15 is not fully cleared but it seems to have a role in regulating inflammatory pathways and to be involved in regulating apoptosis, cell repair and cell growth, which are biological processes observed in cardiovascular and neoplastic disorders. GDF-15 has shown to be a strong prognostic protein in patients with different diseases such as heart diseases and cancer. |
| Immunogen | KLH conjugated synthetic peptide derived from human GDF-15 221-308/308 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | GDF 15
GDF-15 Growth differentiation factor 15
Growth/differentiation factor 15 Macrophage inhibitory cytokine 1 MIC 1 MIC-1 MIC1 NAG 1 NAG1 NRG 1 NRG1 NSAID (nonsteroidal anti inflammatory drug) activated protein 1 NSAID NSAID regulated protein 1 PLAB Placental bone morphogenetic protein Placental bone morphogenic protein Placental TGF beta placental transformation growth factor beta Prostate differentiation factor PTGF beta PTGFB prostate derived factor. |
|---|---|
| Molecular Weight(MW) | 12.5/34 KDa |
| Cellular Localization | Secreted. |
| SwissProt ID | Q99988 |
|---|
Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Mouse placenta); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (GDF15) Polyclonal Antibody, Unconjugated (ER1909-46) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
Application
Fig2: Sample: K562 Cell Lysate at 30 ug A431 Cell Lysate at 30 ug Primary: Anti-GDF15 (ER1909-46) at 1:300 dilution; Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000 dilution; Predicted band size:34 kD Observed band size:30 kD| Positive Control | Highly expressed in placenta, with lower levels in prostate and colon and some expression in kidney. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
