Application
Fig1: Sample: Cerebrum (Mouse) Lysate at 40 ug Primary: Anti- GEP100/IQSEC1 (ER1909-49) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 108 kD Observed band size: 108 kD| Product Name | GEP100/IQSEC1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ADP-ribosylation factors guanine nucleotide-exchange protein 100 ADP-ribosylation factors guanine nucleotide-exchange protein 2 ARF GEP100 ARFGEP100 BRAG2 Brefeldin A resistant ARF GEF2 Brefeldin-resistant Arf-GEF 2 protein GEP100 IQ motif and Sec7 domain 1 IQ motif and SEC7 domain-containing protein 1 IQEC1_HUMAN Iqsec1 Kiaa0763. |
| Product description | In addition to accelerate GTP gamma S binding by ARFs of all three classes, it appears to function preferentially as a guanine nucleotide exchange protein for ARF6, mediating internalisation of beta-1 integrin. |
| Immunogen | KLH conjugated synthetic peptide derived from human GEP100 451-550/963 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatDogPigHorseRabbitSheep |
| Concentration | 1mg/ml |
| Alternative Names | ADP-ribosylation factors guanine nucleotide-exchange protein 100 ADP-ribosylation factors guanine nucleotide-exchange protein 2 ARF GEP100 ARFGEP100 BRAG2 Brefeldin A resistant ARF GEF2 Brefeldin-resistant Arf-GEF 2 protein GEP100 IQ motif and Sec7 domain 1 IQ motif and SEC7 domain-containing protein 1 IQEC1_HUMAN Iqsec1 Kiaa0763. |
|---|---|
| Molecular Weight(MW) | 108 KDa |
| Cellular Localization | Cytoplasm. Nucleus. At steady state, may be preferentially cytosolic. |
| SwissProt ID | Q6DN90 |
|---|
Application
Fig1: Sample: Cerebrum (Mouse) Lysate at 40 ug Primary: Anti- GEP100/IQSEC1 (ER1909-49) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 108 kD Observed band size: 108 kD| Positive Control | Expressed in brain, ovary, heart, lung, liver, kidney and leukocytes. Moderate expression was also detected in lung, skeletal muscle, placenta, small intestine, pancreas, spleen and testis. |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
