Application
Fig1: Protein: A549(human) lyates at 40ug; Primary: Rabbit Anti-GFM2 (ER1909-51) at 1:300; Secondary: 800CW Conjugated Goat (polyclonal) Anti-Rabbit IgG(H+L) at 1: 10000; Predicted band size:87 kD Observed band size:63 kD| Product Name | GFM2 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | EF-G2mt EFG2 EFG2mt elongation factor G 2, mitochondrial Elongation factor G2 G elongation factor mitochondrial 2 GFM2 hEFG2 mEFG 2 mEFG2 Mitochondrial elongation factor G2 mitochondrial ribosome recycling factor 2 MRRF2 MST027 MSTP027 OTTHUMP00000222951 OTTHUMP00000222952 ribosome-releasing factor 2, mitochondrial RRF2 RRF2mt. |
| Product description | Mitochondrial GTPase that mediates the disassembly of ribosomes from messenger RNA at the termination of mitochondrial protein biosynthesis. Acts in collaboration with MRRF. GTP hydrolysis follows the ribosome disassembly and probably occurs on the ribosome large subunit. Not involved in the GTP-dependent ribosomal translocation step during translation elongation. |
| Immunogen | KLH conjugated synthetic peptide derived from human GFM2 151-250/779 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatDogPigCowSheep |
| Concentration | 1mg/ml |
| Alternative Names | EF-G2mt
EFG2
EFG2mt
elongation factor G 2 mitochondrial Elongation factor G2 G elongation factor mitochondrial 2 GFM2 hEFG2 mEFG 2 mEFG2 Mitochondrial elongation factor G2 mitochondrial ribosome recycling factor 2 MRRF2 MST027 MSTP027 OTTHUMP00000222951 OTTHUMP00000222952 ribosome-releasing factor 2 mitochondrial RRF2 RRF2mt. |
|---|---|
| Molecular Weight(MW) | 87 KDa |
| Cellular Localization | Mitochondrial. |
| SwissProt ID | Q969S9 |
|---|
Application
Fig1: Protein: A549(human) lyates at 40ug; Primary: Rabbit Anti-GFM2 (ER1909-51) at 1:300; Secondary: 800CW Conjugated Goat (polyclonal) Anti-Rabbit IgG(H+L) at 1: 10000; Predicted band size:87 kD Observed band size:63 kD| Positive Control | Widely expressed. |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
