Application
Fig1: Sample: Hela(Human) Cell Lysate at 30 ug HepG2(Human) Cell Lysate at 30 ug Primary: Anti-Gliomedin (ER1909-65) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 59 kD Observed band size: 59 kDApplication
Fig2: Sample: B16(Mouse) Cell Lysate at 30 ug Primary: Anti-Gliomedin (ER1909-65) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 59 kD Observed band size: 59 kDApplication
Fig3: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Gliomedin) Polyclonal Antibody, Unconjugated (ER1909-65) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig4: Sample: Cerebrum (Mouse) Lysate at 40 ug Primary: Anti-Gliomedin (ER1909-65) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 59 kD Observed band size: 59 kDApplication
Fig5: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Gliomedin) Polyclonal Antibody, Unconjugated (ER1909-65) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Product Name | Gliomedin |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CANCER RELATED GENE LIVER 2 Cancer related gene-Liver 2 CLOM collomin Colm (gene name) COLM Colmedin CRG L2 FLJ23917 Gldn (gene name) UNC 112 GLDN_HUMAN. |
| Product description | Gliomedin is a member of the collagen superfamily, it is a glial ligand for neurofascin and NrCAM, two axonal immunoglobulin cell adhesion molecules that are associated with Na+ channels at the nodes of Ranvier. Gliomedin provides a glial cue for the formation of peripheral nodes of Ranvier. Gliomedin is expressed by myelinating Schwann cells and accumulates at the edges of each myelin segment during development, where it aligns with the forming nodes of ranvier. Eliminating the expression of gliomedin or the addition of a soluble extracellular domain of neurofascin to myelinating cultures abolishes node formation. Gliomedin is expressed in the PNS nodes of ranvier, but not in the CNS nodes of ranvier. Gliomedin also displays high expression in murine and human hepatocellular carcinomas (HCC). Its restricted expression in normal tissues and unique early upregulation during tumor development make it an excellent candidate as a new clinical marker of HCC. |
| Immunogen | KLH conjugated synthetic peptide derived from human Gliomedin/COLM 365-460/551 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatDogPigCowHorseSheep |
| Concentration | 1mg/ml |
| Alternative Names | CANCER RELATED GENE LIVER 2 Cancer related gene-Liver 2 CLOM collomin Colm (gene name) COLM Colmedin CRG L2 FLJ23917 Gldn (gene name) UNC 112 GLDN_HUMAN. |
|---|---|
| Molecular Weight(MW) | 59 KDa |
| Cellular Localization | Cell membrane; Single-pass type II membrane protein. Note=Localizes to the nodes of Ranvier. |
| SwissProt ID | Q6ZMI3 |
|---|
Application
Fig1: Sample: Hela(Human) Cell Lysate at 30 ug HepG2(Human) Cell Lysate at 30 ug Primary: Anti-Gliomedin (ER1909-65) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 59 kD Observed band size: 59 kD
Application
Fig2: Sample: B16(Mouse) Cell Lysate at 30 ug Primary: Anti-Gliomedin (ER1909-65) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 59 kD Observed band size: 59 kD
Application
Fig3: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Gliomedin) Polyclonal Antibody, Unconjugated (ER1909-65) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig4: Sample: Cerebrum (Mouse) Lysate at 40 ug Primary: Anti-Gliomedin (ER1909-65) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 59 kD Observed band size: 59 kD
Application
Fig5: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Gliomedin) Polyclonal Antibody, Unconjugated (ER1909-65) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Positive Control | Specifically expressed in spinal cord, brain, placenta and sciatic nerve. More abundant in peripheral than central nervous system. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
