Application
Fig1: Protein: rat liver lysates, 30ug; Primary: Anti-GLK(ER1909-66) at 1:200; Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000; ECL excitated the fluorescence; Predicted band size : 51kD Observed band size : 51kDApplication
Fig2: Blank control: A431. Primary Antibody (green line): Rabbit Anti-GLK antibody (ER1909-66) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody: Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 20% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at -20℃ .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.Application
Fig3: Sample: Panc-1(Human) Cell Lysate at 30 ug Primary: Anti-GLK (ER1909-66) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 51 kD Observed band size: 51 kD| Product Name | GLK |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Glucokinase ATP D hexose 6 phosphotransferase GCK GK Glucokinase isoform 1 Hexokinase 4 Hexokinase D Hexokinase D pancreatic isozyme Hexokinase type IV Hexokinase4 HexokinaseD HHF 3 HHF3 HK 4 HK IV HK4 HXKP Maturity onset diabetes of the young 2 MODY 2 MODY2 GLUKA RNGK2 HXK4_HUMAN. |
| Product description | Catalyzes the initial step in utilization of glucose by the beta-cell and liver at physiological glucose concentration. Glucokinase has a high Km for glucose, and so it is effective only when glucose is abundant. The role of GCK is to provide G6P for the synthesis of glycogen. Pancreatic glucokinase plays an important role in modulating insulin secretion. Hepatic glucokinase helps to facilitate the uptake and conversion of glucose by acting as an insulin-sensitive determinant of hepatic glucose usage. |
| Immunogen | KLH conjugated synthetic peptide derived from human GCK 101-200/465 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:500-2000: FC:3ug/Test: |
|
| Species Reactivity | HumanMouseRatChickenDogPigHorseRabbit |
| Concentration | 1mg/ml |
| Alternative Names | Glucokinase ATP D hexose 6 phosphotransferase GCK GK Glucokinase isoform 1 Hexokinase 4 Hexokinase D Hexokinase D pancreatic isozyme Hexokinase type IV Hexokinase4 HexokinaseD HHF 3 HHF3 HK 4 HK IV HK4 HXKP Maturity onset diabetes of the young 2 MODY 2 MODY2 GLUKA RNGK2 HXK4_HUMAN. |
|---|---|
| Molecular Weight(MW) | 51 KDa |
| SwissProt ID | P35557 |
|---|
Application
Fig1: Protein: rat liver lysates, 30ug; Primary: Anti-GLK(ER1909-66) at 1:200; Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000; ECL excitated the fluorescence; Predicted band size : 51kD Observed band size : 51kD
Application
Fig2: Blank control: A431. Primary Antibody (green line): Rabbit Anti-GLK antibody (ER1909-66) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody: Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 20% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at -20℃ .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Application
Fig3: Sample: Panc-1(Human) Cell Lysate at 30 ug Primary: Anti-GLK (ER1909-66) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 51 kD Observed band size: 51 kD| Positive Control | Isoform 1 is expressed in pancreas. Isoform 2 and isoform 3 is expressed in liver. |
|---|---|
| Application Notes | WB:1:500-2000: FC:3ug/Test: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
