Application
Fig1: Sample: Liver（Mouse） lysate at 30 ug; Primary: Anti- GLP-1R (ER1909-68) at 1:300 dilution; Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000 dilution; Predicted band size: 51 kD Observed band size: 51 kDApplication
Fig2: Sample: Cerebrum(Rat) Lysate at 40 ug Primary: Anti-GLP-1R (ER1909-68) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 51 kD Observed band size: 51 kDApplication
Fig3: Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-GLP-1R Polyclonal Antibody, Unconjugated(ER1909-68) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingApplication
Fig4: Tissue/cell: mouse pancreas tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-GLP-1R Polyclonal Antibody, Unconjugated(ER1909-68) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining| Product Name | GLP-1R |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Glucagon like peptide-1 receptor GLP 1 R GLP 1 receptor GLP 1R Glp1r GLP glp1r Glucagon like peptide 1 receptor RATGL1RCP Glip MGC138331 GLP-1-R GLP1R_HUMAN Glucagon-like peptide 1 receptor GLP-1 receptor GLP-1-R GLP-1R Precursor. |
| Product description | The glucagon-like peptide-1 receptor (GLP1R) is a receptor protein found on beta cells of the pancreas. It is involved in the control of blood sugar level by enhancing insulin secretion. In humans it is synthesised by the gene GLP1R, which is present on chromosome 6. It is a member of the glucagon receptor family of G protein-coupled receptors. GLP1R is composed of two domains, one extracellular (ECD) that binds the C-terminal helix of GLP-1, and one transmembrane (TMD) domain[9] that binds the N-terminal region of GLP-1. In the TMD domain there is a fulcrum of polar residues that regulates the biased signaling of the receptor while the transmembrane helical boundaries and extracellular surface are a trigger for biased agonism. |
| Immunogen | KLH conjugated synthetic peptide derived from human GLP-1R 101-200/463 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatDog |
| Concentration | 1mg/ml |
| Alternative Names | Glucagon like peptide-1 receptor GLP 1 R GLP 1 receptor GLP 1R Glp1r GLP glp1r Glucagon like peptide 1 receptor RATGL1RCP Glip MGC138331 GLP-1-R GLP1R_HUMAN Glucagon-like peptide 1 receptor GLP-1 receptor GLP-1-R GLP-1R Precursor. |
|---|---|
| Molecular Weight(MW) | 51 KDa |
| Cellular Localization | Cell membrane; Multi-pass membrane protein. |
| SwissProt ID | O35659 |
|---|
Application
Fig1: Sample: Liver（Mouse） lysate at 30 ug; Primary: Anti- GLP-1R (ER1909-68) at 1:300 dilution; Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000 dilution; Predicted band size: 51 kD Observed band size: 51 kD
Application
Fig2: Sample: Cerebrum(Rat) Lysate at 40 ug Primary: Anti-GLP-1R (ER1909-68) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 51 kD Observed band size: 51 kD
Application
Fig3: Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-GLP-1R Polyclonal Antibody, Unconjugated(ER1909-68) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Application
Fig4: Tissue/cell: mouse pancreas tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-GLP-1R Polyclonal Antibody, Unconjugated(ER1909-68) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining| Positive Control | Liver(Mouse) lysate, Cerebrum(Rat) Lysate, rat brain tissue, mouse pancreas tissue. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
