Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Glucagon Receptor) Polyclonal Antibody, Unconjugated (ER1909-70) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (human stomach tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Glucagon Receptor) Polyclonal Antibody, Unconjugated (ER1909-70) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig3: Blank control(blue): Molt-4 Cells(fixed with 2% paraformaldehyde (10 min)). Primary Antibody: Rabbit Anti-Glucagon Receptor/AF647 Conjugated antibody (ER1909-70/AF647), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/AF647(orange) ,used under the same conditions.Application
Fig4: Sample: Cerebellum (Mouse) Lysate at 40 ug Primary: Anti-Glucagon Receptor (ER1909-70) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 51 kD Observed band size: 51 kDApplication
Fig5: Sample:Kidney (Mouse) Lysate at 40 ug Primary: Anti-Glucagon Receptor(ER1909-70)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 51kD Observed band size: 51kD| Product Name | Glucagon Receptor |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GCGR GGR GL R GL-R GLR GLR_HUMAN MGC138246 Glucagon receptor precursor. |
| Product description | This is a receptor for glucagon which plays a central role in regulating the level of blood glucose by controlling the rate of hepatic glucose production and insulin secretion. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase and also a phosphatidylinositol-calcium second messenger system. |
| Immunogen | KLH conjugated synthetic peptide derived from human Glucagon Receptor 11-110/477 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-PFC |
| WB:1:500-2000: IHC-P:1:400-800: FC:1μg/Test: |
|
| Species Reactivity | HumanMouseRatChickenDogPigCowGuinea Pig |
| Concentration | 1mg/ml |
| Alternative Names | GCGR GGR GL R GL-R GLR GLR_HUMAN MGC138246 Glucagon receptor precursor. |
|---|---|
| Molecular Weight(MW) | 51 KDa |
| Cellular Localization | Cell membrane; Multi-pass membrane protein. |
| SwissProt ID | P47871 |
|---|
Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Glucagon Receptor) Polyclonal Antibody, Unconjugated (ER1909-70) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (human stomach tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Glucagon Receptor) Polyclonal Antibody, Unconjugated (ER1909-70) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig3: Blank control(blue): Molt-4 Cells(fixed with 2% paraformaldehyde (10 min)). Primary Antibody: Rabbit Anti-Glucagon Receptor/AF647 Conjugated antibody (ER1909-70/AF647), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/AF647(orange) ,used under the same conditions.
Application
Fig4: Sample: Cerebellum (Mouse) Lysate at 40 ug Primary: Anti-Glucagon Receptor (ER1909-70) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 51 kD Observed band size: 51 kD
Application
Fig5: Sample:Kidney (Mouse) Lysate at 40 ug Primary: Anti-Glucagon Receptor(ER1909-70)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 51kD Observed band size: 51kD| Positive Control | Expressed in fetal tissues, including testis, adrenal gland, brain, white blood cells and brain. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: FC:1μg/Test: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
