Application
Fig1: Sample: K562 Cell (Human) Lysate at 30 ug Primary: Anti- GLUR4 (ER1909-72)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 98kD Observed band size: 118kDApplication
Fig2: Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (GLUR4) Polyclonal Antibody, Unconjugated (ER1909-72) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Product Name | GLUR4 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Ionotropic Glutamate receptor 4 AMPA 4 AMPA selective glutamate receptor 4 GluR 4 GluR D GLUR4 GLUR4C GLURD Glutamate receptor 4 Glutamate receptor ionotrophic AMPA 4 GRIA 4 GRIA4 GRIA4_HUMAN. |
| Product description | Receptor for glutamate that functions as ligand-gated ion channel in the central nervous system and plays an important role in excitatory synaptic transmission. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. In the presence of CACNG4 or CACNG7 or CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of glutamate. |
| Immunogen | KLH conjugated synthetic peptide derived from human GLUR4 501-600/902 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatDogPigCow |
| Concentration | 1mg/ml |
| Alternative Names | Ionotropic Glutamate receptor 4 AMPA 4 AMPA selective glutamate receptor 4 GluR 4 GluR D GLUR4 GLUR4C GLURD Glutamate receptor 4 Glutamate receptor ionotrophic AMPA 4 GRIA 4 GRIA4 GRIA4_HUMAN. |
|---|---|
| Molecular Weight(MW) | 98 KDa |
| Cellular Localization | Cell membrane; Multi-pass membrane protein.Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Cell projection, dendrite. Note=Interaction with CNIH2, CNIH3 and PRKCG promotes cell surface expression (By similarity). |
| SwissProt ID | P48058 |
|---|
Application
Fig1: Sample: K562 Cell (Human) Lysate at 30 ug Primary: Anti- GLUR4 (ER1909-72)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 98kD Observed band size: 118kD
Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (GLUR4) Polyclonal Antibody, Unconjugated (ER1909-72) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Positive Control | Detected in cerebellum (at protein level). |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
