Application
Fig1: Sample: Heart (Rat) Lysate at 40 ug Primary: Anti- GLUT12 (ER1909-73)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 68kD Observed band size: 68kDApplication
Fig2: Paraformaldehyde-fixed, paraffin embedded (rat stomach tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (GLUT12) Polyclonal Antibody, Unconjugated (ER1909-73) at 1:400 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.| Product Name | GLUT12 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GLUT-12 GTR12_HUMAN Glucose transporter type 12 Glucose transporter type 12 GLUT 12 GLUT8 SLC2A12 Solute carrier family 2 (facilitated glucose transporter) member 12 Solute carrier family 2 facilitated glucose transporter member 12 Solute carrier family 2 member 12. |
| Product description | Facilitative glucose transporter. |
| Immunogen | KLH conjugated synthetic peptide derived from human GLUT12 251-350/617 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-PFC |
| WB:1:500-2000: IHC-P:1:400-800: FC:2μg/Test: |
|
| Species Reactivity | HumanMouseRatChickenDogPigCowHorseRabbitGuinea Pig |
| Concentration | 1mg/ml |
| Alternative Names | GLUT-12 GTR12_HUMAN Glucose transporter type 12 Glucose transporter type 12 GLUT 12 GLUT8 SLC2A12 Solute carrier family 2 (facilitated glucose transporter) member 12 Solute carrier family 2 facilitated glucose transporter member 12 Solute carrier family 2 member 12. |
|---|---|
| Molecular Weight(MW) | 68 KDa |
| Cellular Localization | Endomembrane system; Multi-pass membrane protein. Cytoplasm, perinuclear region. Note=Localizes primarily perinuclear region in the absence of insulin. |
| SwissProt ID | Q8TD20 |
|---|
Application
Fig1: Sample: Heart (Rat) Lysate at 40 ug Primary: Anti- GLUT12 (ER1909-73)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 68kD Observed band size: 68kD
Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (rat stomach tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (GLUT12) Polyclonal Antibody, Unconjugated (ER1909-73) at 1:400 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.| Positive Control | Predominantly expressed in skeletal muscle, heart and prostate, with lower levels in brain, placenta and kidney. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: FC:2μg/Test: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
