Application
Fig1: Sample: U251(Human) Cell Lysate at 30 ug Primary: Anti-GPM6B (ER1909-87) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 29 kD Observed band size: 34 kDApplication
Fig2: Blank control: RSC96(blue), the cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice. Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 0.2μg in 100 μL1X PBS containing 0.5% BSA(green).Application
Fig3: Sample: Brain (Mouse) Lysate at 40 ug Primary: Anti-GPM6B (ER1909-87) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 29 kD Observed band size: 36 kD| Product Name | GPM6B |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | M6b Neuronal membrane glycoprotein M6b rhombex 29 GPM6B_HUMAN. |
| Product description | May be involved in neural development. Involved in regulation of osteoblast function and bone formation. Involved in matrix vesicle release by osteoblasts; this function seems to involve maintenance of the actin cytoskeleton. May be involved in cellular trafficking of SERT and thereby in regulation of serotonin uptake. |
| Immunogen | KLH conjugated synthetic peptide derived from human GPM6B 1-100/265 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:500-2000: FC:1μg/Test: |
|
| Species Reactivity | HumanMouseRatPigHorse |
| Concentration | 1mg/ml |
| Alternative Names | M6b Neuronal membrane glycoprotein M6b rhombex 29 GPM6B_HUMAN. |
|---|---|
| Molecular Weight(MW) | 29 KDa |
| Cellular Localization | Cell membrane; Multi-pass membrane protein (By similarity). Note=Colocalizes with SERT at the plasma membrane |
| SwissProt ID | Q13491 |
|---|
Application
Fig1: Sample: U251(Human) Cell Lysate at 30 ug Primary: Anti-GPM6B (ER1909-87) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 29 kD Observed band size: 34 kD
Application
Fig2: Blank control: RSC96(blue), the cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice. Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 0.2μg in 100 μL1X PBS containing 0.5% BSA(green).
Application
Fig3: Sample: Brain (Mouse) Lysate at 40 ug Primary: Anti-GPM6B (ER1909-87) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 29 kD Observed band size: 36 kD| Positive Control | Neurons and glia; cerebellar Bergmann glia, in glia within white matter tracts of the cerebellum and cerebrum, and in embryonic dorsal root ganglia. |
|---|---|
| Application Notes | WB:1:500-2000: FC:1μg/Test: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
