Application
Fig1: Blank control(blue): Mouse Kidney Cells(fixed with 2% paraformaldehyde (10 min)).
Primary Antibody:Rabbit Anti- GPR110 antibody(ER1909-93), Dilution: 1ug in 100 uL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Application
Fig2: Sample: A431 (human)Cell Lysate at 40 ug
Primary: Anti-GPR110(ER1909-93) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 99 kD
Observed band size: 109 kD
Application
Fig3: Sample: A549 (human)Cell Lysate at 40 ug
Primary: Anti-GPR110(ER1909-93) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 99 kD
Observed band size: 109 kD