Application
Fig1: Blank control (blue line): Mouse colon (blue). Primary Antibody (green line): Rabbit Anti-GPR125 antibody (ER1909-97) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): F(ab’)2 fragment goat anti-rabbit IgG-FITC Dilution: 1μg /test. Protocol The cells were fixed with 2% paraformaldehyde for 10 min at room temperatureCells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.Application
itrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (GPR125) Polyclonal Antibody, Unconjugated (ER1909-97) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig3: Sample: Spinal cord (Mouse) Lysate at 40 ug Primary: Anti- GPR125 (ER1909-97) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 143 kD Observed band size: 183 kD| Product Name | GPR125 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | FLJ38547 G protein coupled receptor 125 PGR21 Probable G protein coupled receptor 125 precursor TEM5 like TEM5L GP125_HUMAN GPCR125. |
| Product description | GPR125 is an orphan receptor which has a leucine rich repeat (LRR),an immunoglobulin (Ig) domain, and a hormone-binding domain (HBD). The Ig domain shows similarities to motilin andtitin, while the LRR domain shows similarities to LRIG1 and SLIT1-2. ESTs have been isolated primarily from amnion,connective tissue, ear, embryo, eye,ganglion, heart, lung,placenta, and skin libraries. |
| Immunogen | KLH conjugated synthetic peptide derived from human GPR125 422-530/1321 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-PFC |
| WB:1:500-2000: IHC-P:1:400-800: FC:1μg/Test: |
|
| Species Reactivity | HumanMouseRatDogCowHorseSheep |
| Concentration | 1mg/ml |
| Alternative Names | FLJ38547 G protein coupled receptor 125 PGR21 Probable G protein coupled receptor 125 precursor TEM5 like TEM5L GP125_HUMAN GPCR125. |
|---|---|
| Molecular Weight(MW) | 143 KDa |
| Cellular Localization | Cell membrane; Multi pass membrane protein. |
| SwissProt ID | Q8IWK6 |
|---|
Application
Fig1: Blank control (blue line): Mouse colon (blue). Primary Antibody (green line): Rabbit Anti-GPR125 antibody (ER1909-97) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): F(ab’)2 fragment goat anti-rabbit IgG-FITC Dilution: 1μg /test. Protocol The cells were fixed with 2% paraformaldehyde for 10 min at room temperatureCells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Application
itrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (GPR125) Polyclonal Antibody, Unconjugated (ER1909-97) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig3: Sample: Spinal cord (Mouse) Lysate at 40 ug Primary: Anti- GPR125 (ER1909-97) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 143 kD Observed band size: 183 kD| Application Notes | WB:1:500-2000: IHC-P:1:400-800: FC:1μg/Test: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
