Application
Fig1: Blank control: RSC96 Cells(blue). Primary Antibody: Rabbit Anti-GPR55/AF488 Conjugated antibody (ER1910-10-AF488), Dilution: 5μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/AF488(orange) ,used under the same conditions.Application
Fig2: Sample: K562 Cell Lysate at 40 ug Primary: Anti- GPR55 (ER1910-10) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 37 kD Observed band size: 35 kDApplication
Fig3: Sample: Kidney (Mouse) Lysate at 40 ug Primary: Anti-GPR55 (ER1910-10) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 37 kD Observed band size: 37 kD| Product Name | GPR55 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GPCR GPR55 G protein coupled receptor 55 G protein-coupled receptor 55 G protein-coupled receptor 55 GPCR GPR55 GPR 55 GPR55 Probable G protein coupled receptor 55 GPR55_HUMAN. |
| Product description | May be involved in hyperalgesia associated with inflammatory and neuropathic pain. Receptor for L-alpha-lysophosphatidylinositol (LPI). LPI induces Ca(2+) release from intracellular stores via the heterotrimeric G protein GNA13 and RHOA. Putative cannabinoid receptor. May play a role in bone physiology by regulating osteoclast number and function. |
| Immunogen | KLH conjugated synthetic peptide derived from human GPR55 141-240/319 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:500-2000: FC:2ug/Test: |
|
| Species Reactivity | HumanMouseRatChickenDogHorseRabbit |
| Concentration | 1mg/ml |
| Alternative Names | GPCR GPR55 G protein coupled receptor 55 G protein-coupled receptor 55 G protein-coupled receptor 55 GPCR GPR55 GPR 55 GPR55 Probable G protein coupled receptor 55 GPR55_HUMAN. |
|---|---|
| Molecular Weight(MW) | 37 KDa |
| Cellular Localization | Cell membrane; Multi-pass membrane protein. |
| SwissProt ID | Q9Y2T6 |
|---|
Application
Fig1: Blank control: RSC96 Cells(blue). Primary Antibody: Rabbit Anti-GPR55/AF488 Conjugated antibody (ER1910-10-AF488), Dilution: 5μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/AF488(orange) ,used under the same conditions.
Application
Fig2: Sample: K562 Cell Lysate at 40 ug Primary: Anti- GPR55 (ER1910-10) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 37 kD Observed band size: 35 kD
Application
Fig3: Sample: Kidney (Mouse) Lysate at 40 ug Primary: Anti-GPR55 (ER1910-10) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 37 kD Observed band size: 37 kD| Positive Control | Expressed in the caudate nucleus and putamen, but not detected in the hippocampus, thalamus, pons cerebellum, frontal cortex of the brain or in the liver. Expressed in osteoclasts and osteoblasts. |
|---|---|
| Application Notes | WB:1:500-2000: FC:2ug/Test: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
