Application
Fig1: Sample: A549 Cell (Human) Lysate at 30 ug Primary: Anti-GPR78(ER1910-15)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 39 kD Observed band size: 39 kDApplication
Fig2: Blank control: 293T Cells(blue). Primary Antibody: Rabbit Anti-GPR78/AF488 Conjugated antibody (ER1910-15-AF488), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/AF488(orange) ,used under the same conditions. Protocol The cells were fixed with 2% paraformaldehyde (10 min) . The cells were washed twice with 1 X PBS. The cells were incubated in 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions followed by the incubated with antibody (ER1910-15-AF488, 1μg /1x10^6 cells) for 30 min on ice. Acquisition of 20,000 events was performed.Application
Fig3: Blank control (blue line): U937 (blue). Primary Antibody (green line): Rabbit Anti-GPR7 antibody (ER1910-15) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells were fixed with 2% paraformaldehyde for 10 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.| Product Name | GPR78 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GPCR GPR78 G protein-coupled receptor 78 G protein coupled receptor GPR78 G-protein coupled receptor 78 GPR78 GPR78_HUMAN PRO19818 UNQ5925. |
| Product description | Orphan receptor. Displays a significant level of constitutive activity. Its effect is mediated by G(s)-alpha protein that stimulate adenylate cyclase, resulting in an elevation of intracellular cAMP. |
| Immunogen | KLH conjugated synthetic peptide derived from human GPR78 201-300/363 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:500-2000: FC:1μg /test: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | GPCR GPR78 G protein-coupled receptor 78 G protein coupled receptor GPR78 G-protein coupled receptor 78 GPR78 GPR78_HUMAN PRO19818 UNQ5925. |
|---|---|
| Molecular Weight(MW) | 39 KDa |
| Cellular Localization | Cell membrane; Multi-pass membrane protein. |
| SwissProt ID | Q96P69 |
|---|
Application
Fig1: Sample: A549 Cell (Human) Lysate at 30 ug Primary: Anti-GPR78(ER1910-15)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 39 kD Observed band size: 39 kD
Application
Fig2: Blank control: 293T Cells(blue). Primary Antibody: Rabbit Anti-GPR78/AF488 Conjugated antibody (ER1910-15-AF488), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/AF488(orange) ,used under the same conditions. Protocol The cells were fixed with 2% paraformaldehyde (10 min) . The cells were washed twice with 1 X PBS. The cells were incubated in 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions followed by the incubated with antibody (ER1910-15-AF488, 1μg /1x10^6 cells) for 30 min on ice. Acquisition of 20,000 events was performed.
Application
Fig3: Blank control (blue line): U937 (blue). Primary Antibody (green line): Rabbit Anti-GPR7 antibody (ER1910-15) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells were fixed with 2% paraformaldehyde for 10 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.| Positive Control | High level of expression in placenta. Expressed throughout the brain at low level. No expression detected in skeletal muscle, lung, heart, liver, pancreas, or kidney. |
|---|---|
| Application Notes | WB:1:500-2000: FC:1μg /test: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
