Application
Fig1: Sample: SP2/0 (mouse)Cell Lysate at 40 ug Lung(mouse) Lysate at 40 ug HL-60 (human)Cell Lysate at 40 ug Muscle (mouse) Lysate at 40 ug Primary: Anti- GPR84 (ER1910-16)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 44kD Observed band size: 44 kDApplication
Fig2: Sample: bone (Rat) Lysate at 40 ug Primary: Anti- GPR84 (ER1910-16)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 44kD Observed band size: 44 kD| Product Name | GPR84 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GPCR EX33 EX 33 EX33 G protein coupled receptor 84 G protein coupled receptor EX 33 G protein coupled receptor EX33 G-protein coupled receptor 84 GPCR 4 GPCR4 GPR 84 GPR84 GPR84_HUMAN Inflammation related G protein coupled receptor EX 33 Inflammation related G protein coupled receptor EX33 Inflammation-related G-protein coupled receptor EX33 GPCR GPR84. |
| Product description | Receptor for medium-chain free fatty acid (FFA) with carbon chain lengths of C9 to C14. Capric acid (C10:0), undecanoic acid (C11:0) and lauric acid (C12:0) are the most potent agonists. Not activated by short-chain and long-chain saturated and unsaturated FFAs. Activation by medium-chain free fatty acid is coupled to a pertussis toxin sensitive G(i/o) protein pathway. May have important roles in processes from fatty acid metabolism to regulation of the immune system. |
| Immunogen | KLH conjugated synthetic peptide derived from human GPCR EX33/GPR84 1-100/396 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:500-2000: FC:3ug/Test: |
|
| Species Reactivity | HumanMouseRatDogCowHorseSheepMonkey |
| Concentration | 1mg/ml |
| Alternative Names | GPCR EX33 EX 33 EX33 G protein coupled receptor 84 G protein coupled receptor EX 33 G protein coupled receptor EX33 G-protein coupled receptor 84 GPCR 4 GPCR4 GPR 84 GPR84 GPR84_HUMAN Inflammation related G protein coupled receptor EX 33 Inflammation related G protein coupled receptor EX33 Inflammation-related G-protein coupled receptor EX33 GPCR GPR84. |
|---|---|
| Molecular Weight(MW) | 44 KDa |
| Cellular Localization | Cell membrane. |
| SwissProt ID | Q9NQS5 |
|---|
Application
Fig1: Sample: SP2/0 (mouse)Cell Lysate at 40 ug Lung(mouse) Lysate at 40 ug HL-60 (human)Cell Lysate at 40 ug Muscle (mouse) Lysate at 40 ug Primary: Anti- GPR84 (ER1910-16)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 44kD Observed band size: 44 kD
Application
Fig2: Sample: bone (Rat) Lysate at 40 ug Primary: Anti- GPR84 (ER1910-16)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 44kD Observed band size: 44 kD| Positive Control | Expressed predominantly in hematopoietic tissues. High levels detected in the bone marrow and lower levels in the peripheral leukocytes and lung. Also expressed in brain, heart, muscle, colon, thymus, spleen, kidney, liver, placenta and intestine. Within the leukocyte population expression is higher in neutrophils and eosinophils relative to T- or B-lymphocytes. |
|---|---|
| Application Notes | WB:1:500-2000: FC:3ug/Test: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
