Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (GPR89A) Polyclonal Antibody, Unconjugated (ER1910-17) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Product Name | GPR89A |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CGI-13 Golgi pH regulator A Golgi pH regulator B GPHR GPHRA GPHRB GPR89 GPR89A GPR89B HSPC201 Protein GPR89A Protein GPR89B Putative MAPK-activating protein PM01 Putative NF-kappa-B-activating protein 90 SH120 UNQ192/PRO218 GPHRA_HUMAN. |
| Product description | Voltage dependent anion channel required for acidification and functions of the Golgi apparatus that may function in counter-ion conductance. |
| Immunogen | KLH conjugated synthetic peptide derived from human GPR89A 221-320/445 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatDogCowRabbitSheep |
| Concentration | 1mg/ml |
| Alternative Names | CGI-13 Golgi pH regulator A Golgi pH regulator B GPHR GPHRA GPHRB GPR89 GPR89A GPR89B HSPC201 Protein GPR89A Protein GPR89B Putative MAPK-activating protein PM01 Putative NF-kappa-B-activating protein 90 SH120 UNQ192/PRO218 GPHRA_HUMAN. |
|---|---|
| Molecular Weight(MW) | 53 KDa |
| Cellular Localization | Golgi apparatus membrane; Multi-pass membrane protein. |
| SwissProt ID | B7ZAQ6 |
|---|
Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (GPR89A) Polyclonal Antibody, Unconjugated (ER1910-17) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Positive Control | Ubiquitous. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
