Application
Fig1: Sample: Liver (Mouse) Lysate at 40 ug Primary: Anti-GPX2 (ER1910-20) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 22 kD Observed band size: 22 kD| Product Name | GPX2 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Gastrointestinal glutathione peroxidase GI GPx Glutathione peroxidase 2 (gastrointestinal) Glutathione peroxidase 2 Glutathione peroxidase gastrointestinal Glutathione peroxidase related protein 2 Glutathione peroxidase-gastrointestinal Glutathione peroxidase-related protein 2 GPRP GPRP-2 GPx 2 GPx-2 GPx-GI GPX2 GPX2_HUMAN GSHPx 2 GSHPx GI GSHPx-2 GSHPx-GI. |
| Product description | Glutathione peroxidase 2 is an enzyme that in humans is encoded by the GPX2 gene. This gene is a member of the glutathione peroxidase family encoding a selenium-dependent glutathione peroxidase that is one of two isoenzymes responsible for the majority of the glutathione-dependent hydrogen peroxide-reducing activity in the epithelium of the gastrointestinal tract. Studies in knockout mice indicate that mRNA expression levels respond to luminal microflora, suggesting a role of the ileal glutathione peroxidases in preventing inflammation in the GI tract. The antioxidant enzyme glutathione peroxidase 2 (Gpx2) is one out of eight known glutathione peroxidases (Gpx1-8) in humans. Mammalian Gpx1, GPx2 (this protein), Gpx3, and Gpx4 have been shown to be selenium-containing enzymes, whereas Gpx6 is a selenoprotein in humans with cysteine-containing homologues in rodents. In selenoproteins, the 21st amino acid selenocysteine is inserted in the nascent polypeptide chain during the process of translational recoding of the UGA stop codon. |
| Immunogen | KLH conjugated synthetic peptide derived from human GPX2/Glutathione Peroxidase 2 41-140/190 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatDogCowHorseRabbitSheep |
| Concentration | 1mg/ml |
| Alternative Names | Gastrointestinal glutathione peroxidase
GI GPx
Glutathione peroxidase 2 (gastrointestinal) Glutathione peroxidase 2 Glutathione peroxidase gastrointestinal Glutathione peroxidase related protein 2 Glutathione peroxidase-gastrointestinal Glutathione peroxidase-related protein 2 GPRP GPRP-2 GPx 2 GPx-2 GPx-GI GPX2 GPX2_HUMAN GSHPx 2 GSHPx GI GSHPx-2 GSHPx-GI. |
|---|---|
| Molecular Weight(MW) | 22 KDa |
| Cellular Localization | Cytoplasm. Mainly cytoplasmic. |
| SwissProt ID | P18283 |
|---|
Application
Fig1: Sample: Liver (Mouse) Lysate at 40 ug Primary: Anti-GPX2 (ER1910-20) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 22 kD Observed band size: 22 kD| Positive Control | Mostly in liver and gastrointestinal tract, not found in heart or kidney. |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
