Application
Fig1: Sample: intestine (Mouse) Lysate at 40 ug skin (Mouse) Lysate at 40 ug Primary: Anti-Gremlin (Bs-1475R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 21 kD Observed band size: 24 kDApplication
Fig2: Sample: intestine (Mouse) Lysate at 40 ug skin (Mouse) Lysate at 40 ug Primary: Anti-Gremlin (Bs-1475R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 21 kD Observed band size: 24 kDApplication
Fig3: Sample: Testis (Mouse) Lysate at 40 ug Primary: Anti- Gremlin (ER1910-23) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 21 kD Observed band size: 21 kD| Product Name | Gremlin |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CKTSF1B1 GREMLIN-1 GREMLIN 1 GREMLIN1 Cysteine knot superfamily BMP antagonist 1 DAND2 DRM GREMLIN Gremlin1 Gremlin 1 Gremlin-1 IHG2 PIG2 proliferation inducing gene 2 protein. |
| Product description | Cytokine that may play an important role during carcinogenesis and metanephric kidney organogenesis, as a BMP antagonist required for early limb outgrowth and patterning in maintaining the FGF4-SHH feedback loop. Down-regulates the BMP4 signaling in a dose-dependent manner. Acts as inhibitor of monocyte chemotaxis. |
| Immunogen | KLH conjugated synthetic peptide derived from human gremlin 101-184/184 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatChickenDogCowHorseRabbit |
| Concentration | 1mg/ml |
| Alternative Names | CKTSF1B1 GREMLIN-1 GREMLIN 1 GREMLIN1 Cysteine knot superfamily BMP antagonist 1 DAND2 DRM GREMLIN Gremlin1 Gremlin 1 Gremlin-1 IHG2 PIG2 proliferation inducing gene 2 protein. |
|---|---|
| Molecular Weight(MW) | 21 KDa |
| Cellular Localization | Secreted. |
| SwissProt ID | O60565 |
|---|
Application
Fig1: Sample: intestine (Mouse) Lysate at 40 ug skin (Mouse) Lysate at 40 ug Primary: Anti-Gremlin (Bs-1475R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 21 kD Observed band size: 24 kD
Application
Fig2: Sample: intestine (Mouse) Lysate at 40 ug skin (Mouse) Lysate at 40 ug Primary: Anti-Gremlin (Bs-1475R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 21 kD Observed band size: 24 kD
Application
Fig3: Sample: Testis (Mouse) Lysate at 40 ug Primary: Anti- Gremlin (ER1910-23) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 21 kD Observed band size: 21 kD| Positive Control | Highly expressed in small intestine, fetal brain and colon. Weakly expressed in brain, ovary, prostate, pancreas and skeletal muscle. In brain found in the region localized around the internal capsule in the large subcortical nuclei, including caudate, putamen, substantia nigra, thalamus and subthalamus. Predominantly expressed in normal cells including neurons, astrocytes and fibroblasts. |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
