Application
Fig1: Sample: Placenta (Mouse) Lysate at 40 ug Primary: Anti-GRF2 (ER1910-24) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 140 kD Observed band size: 120 kDApplication
Fig2: Protein: placenta(mouse) lysate at 40ug; Primary: rabbit Anti-GRF2 (ER1910-24) at 1:300; Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000; Predicted band size: 140 kD Observed band size: 140 kD| Product Name | GRF2 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | C3G C3G protein CRK SH3 binding GNRP CRK SH3-binding GNRP DKFZp781P1719 GRF 2 GRF2 Guanine nucleotide releasing factor 2 (specific for crk proto oncogene) Guanine nucleotide releasing factor 2 Guanine nucleotide-releasing factor 2 OTTHUMP00000064558 Protein C3G Rap guanine nucleotide exchange factor (GEF) 1 Rap guanine nucleotide exchange factor 1 RAPGEF 1 RAPGEF1 RPGF1_HUMAN. |
| Product description | Guanine nucleotide-releasing protein that binds to SH3 domain of CRK and GRB2/ASH. Transduces signals from CRK to activate RAS. |
| Immunogen | KLH conjugated synthetic peptide derived from human GRF2 20-120/1237 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatCowSheep |
| Concentration | 1mg/ml |
| Alternative Names | C3G C3G protein CRK SH3 binding GNRP CRK SH3-binding GNRP DKFZp781P1719 GRF 2 GRF2 Guanine nucleotide releasing factor 2 (specific for crk proto oncogene) Guanine nucleotide releasing factor 2 Guanine nucleotide-releasing factor 2 OTTHUMP00000064558 Protein C3G Rap guanine nucleotide exchange factor (GEF) 1 Rap guanine nucleotide exchange factor 1 RAPGEF 1 RAPGEF1 RPGF1_HUMAN. |
|---|---|
| Molecular Weight(MW) | 140 KDa |
| SwissProt ID | O14827 |
|---|
Application
Fig1: Sample: Placenta (Mouse) Lysate at 40 ug Primary: Anti-GRF2 (ER1910-24) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 140 kD Observed band size: 120 kD
Application
Fig2: Protein: placenta(mouse) lysate at 40ug; Primary: rabbit Anti-GRF2 (ER1910-24) at 1:300; Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000; Predicted band size: 140 kD Observed band size: 140 kD| Positive Control | Ubiquitously expressed in adult and fetus. Expression is high in adult skeletal muscle and placenta and in fetal brain and heart. Low levels of expression in adult and fetal liver. |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
